SRP2018
GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym(s):
E1A
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About This Item
NACRES:
NA.26
UNSPSC Code:
12352202
Biological source:
Saccharomyces cerevisiae, canine
Recombinant:
expressed in E. coli
Assay:
≥70% (SDS-PAGE)
Form:
frozen liquid
Mol wt:
~33 kDa
biological source
Saccharomyces cerevisiae, canine
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
frozen liquid
mol wt
~33 kDa
packaging
pkg of 4 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
100 μg/mL
technique(s)
electrophoretic mobility shift assay: suitable
color
colorless to clear
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
Saccharomyces cerevisiae ... GAL4(855828)
Biochem/physiol Actions
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
A J Berk et al.
Cell, 17(4), 935-944 (1979-08-01)
L Keegan et al.
Science (New York, N.Y.), 231(4739), 699-704 (1986-02-14)
The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription.
T Kodadek
Cellular & molecular biology research, 39(4), 355-360 (1993-01-01)
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5'-CGGN5(T/A)N5CCG-3'. We review the general requirements that must be met for
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