SRP2032
Topo I (C651-765) (C terminal domain) human
recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
别名:
TOPI
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关于此项目
NACRES:
NA.26
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in insect cells
Assay:
≥80% (SDS-PAGE)
Form:
frozen liquid
Mol wt:
~15.2 kDa
biological source
human
recombinant
expressed in insect cells
assay
≥80% (SDS-PAGE)
form
frozen liquid
mol wt
~15.2 kDa
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
500 μg/mL
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TOP1(7150)
Biochem/physiol Actions
Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable