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SRP3164

Sigma-Aldrich

sTRAIL Receptor-1 (DR4) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
Apo2, DR4, TNFRSF10A, soluble TRAIL Receptor-1

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

100 ng/mL

mol wt

22.7 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

O00220

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNFRSF10A(8797)

General description

Soluble tumor necrosis factor-related apoptosis-inducing ligand-receptor 1 (sTRAIL-R1) also known as DR4, is encoded by the gene mapped to human chromosome 8p21-22. Recombinant human soluble TRAIL Receptor-1/DR4 is a 22.7kDa protein (216 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain. TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins. TRAIL-R1 contains a cytoplasmic "death domain," which can activate the cell′s apoptotic machinery. TRAIL Receptor-1/DR4 is activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

Biochem/physiol Actions

Soluble tumor necrosis factor-related apoptosis-inducing ligand-receptor 1 (sTRAIL-R1) interacts with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and stimulate cell-death signaling pathway. Mutation of the sTRAIL-R1 gene is associated with the pathogenesis of non-Hodgkin′s lymphoma (NHL). In addition, variation of TRAIL-R1 also increases the risk of susceptibility to prostate cancer (PCa) in North Indian population.

Sequence

MSGTGAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASQQLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHN

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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The role of TRADD in death receptor signaling.
Pobezinskaya YL and Liu Z.
Cell Cycle, 11(5), 871-876 (2012)
Death receptor 4 variants enhanced prostate cancer risk in North Indian population.
Mittal RD
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 36(7), 5655-5661 (2015)
Somatic mutations of TRAIL-receptor 1 and TRAIL-receptor 2 genes in non-Hodgkin's lymphoma.
Lee SH
Oncogene, 20(3), 399-403 (2001)
Ashkenazi, A., et al.
Cytokine Reference, 1733-1742 (2001)

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