SRP6484
Carbonic Anhydrase 2/CA2 human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
CA-II, CAII, Car2
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352204
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized
Mol wt:
calculated mol wt 30 kDa, observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)
Impurities:
<1 EU/μg endotoxin (LAL test)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 30 kDa, observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)
packaging
pkg of 10 μg, pkg of 50 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CA2(760)
General description
Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.
Application
Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).
Biochem/physiol Actions
Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.
Physical form
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Two novel CAII mutations causing carbonic anhydrase II deficiency syndrome in two unrelated Chinese families.
Pang Q, et al.
Metabolic Brain Disease, 30, 989-989 (2005)
Carbonic anhydrase II: a novel biomarker for pseudomyxoma peritonei.
Jarvinen P, et al.
Acta Pathologica, Microbiologica et Immunological Scandinavica, 125, 207-207 (2017)
A study of anti-carbonic anhydrase II antibodies in rheumatic autoimmune diseases.
Ono M, et al.
Journal of Dermatological Science, 21, 183-183 (1999)
Increased water flux induced by an aquaporin-1/carbonic anhydrase II interaction.
Vilas G, et al.
Molecular Biology of the Cell, 26, 1106-1106 (2015)
Amplification of chromosome 8q21-qter associated with the acquired paclitaxel resistance of nasopharyngeal carcinoma cells.
Li W, et al.
International Journal of Clinical and Experimental Pathology, 8, 12346-12346 (2015)
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