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T9326

Thrombin human

Name: Thrombin human
Brand: SIGMA

BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)

Synonym(s):

Factor IIa

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.21.5 (BRENDA, IUBMB)

MDL number:

MFCD00082072

Specific activity:

≥2,000 units/mg protein

Assay:

≥95% (SDS-PAGE)

Recombinant:

expressed in HEK 293 cells

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Properties

recombinant

expressed in HEK 293 cells

Quality Segment

200

product line

BioUltra

assay

≥95% (SDS-PAGE)

form

aqueous solution

specific activity

≥2,000 units/mg protein

mol wt

37.4 kDa

UniProt accession no.

P00734

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... F2(2147)

Description

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.

Physical form

supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c