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WH0057154M1

Monoclonal Anti-SMURF1 antibody produced in mouse

clone 1D7, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-KIAA1625, Anti-SMAD specific E3 ubiquitin protein ligase 1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
1D7, monoclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
indirect ELISA
indirect immunofluorescence
western blot
Species reactivity:
rat, mouse, human
Citations:
8
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-5 μg/mL
Uniprot accession no.:
Q9HCE7

Key Documents

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Product Name

Monoclonal Anti-SMURF1 antibody produced in mouse, clone 1D7, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1D7, monoclonal

form

buffered aqueous solution

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-5 μg/mL

isotype

IgG2aκ

GenBank accession no.

NM_020429

UniProt accession no.

Q9HCE7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SMURF1(57154)

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Application

Monoclonal Anti-SMURF1 antibody produced in mouse has been used in Western Blotting.

Biochem/physiol Actions

SMAD specific E3 ubiquitin protein ligase 1 (SMURF1) acts as a negative regulator of transforming growth factor β (TGFβ) pathway. It has a role in the nuclear export of mothers against decapentaplegic homolog 7 (SMAD7) and the degradation of SMAD4. During epithelial-mesenchymal transition, SMURF1 dissolves tight junctions in cells. This protein also has a role in cell migration and in the bone morphogenetic protein pathway. SMURF1 has been associated with hepatocellular carcinoma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SMAD specific E3 ubiquitin protein ligase 1 (SMURF1) is an E3 ubiquitin ligase and the gene encoding it is localized on human chromosome 7q22.1.

Immunogen

SMURF1 (NP_065162, 165 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
DSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP

Physical form

Solution in phosphate buffered saline, pH 7.4

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
DC051-1D7
D6241-1D7
09239-1D7
08269-1D7
08144-1D7

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SMURF1 amplification promotes invasiveness in pancreatic cancer.
Kwei KA
PLoS ONE (2011)
Smurf1 controls S phase progression and tumorigenesis through Wee1 degradation.
Wei R
Febs Letters (2017)
Peroxisomal protein PEX13 functions in selective autophagy
Ming Y Lee
EMBO Reports (2016)
Immunolocalization of Smurf1 in Hirano bodies.
Makioka K
Journal of the Neurological Sciences (2014)
Ning Zhang et al.
Journal of clinical and translational hepatology, 12(3), 227-235 (2024-03-01)
Liver iron overload can induce hepatic expression of bone morphogenic protein (BMP) 6 and activate the BMP/SMAD pathway. However, serum iron overload can also activate SMAD but does not induce BMP6 expression. Therefore, the mechanisms through which serum iron overload
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