Anti-AS160/TBC1D4 Antibody, clone 1D6 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1D6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects AS160/TBC1 domain family member 4 (TBC1D4). It targets an epitope within 13 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human TBC1 domain family member 4 (TBC1D4)/AS160)

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell membrane preparation.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected AS160/TBC1D4 in HEK293 cell membrane preparation.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound AS160/TBC1D4 with a KD of 3.6 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected AS160/TBC1D4 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected AS160/TBC1D4 in Caco-2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

TBC1 domain family member 4 (UniProt: O60343; also known as Akt substrate of 160 kDa, AS160) is encoded by the TCB1D4 (also known as AS160, KIAA0603) gene (Gene ID: 9882) in human. AS160 is a widely expressed cytoplasmic protein that acts as a GTPase-activating protein for several Rab proteins. It is phosphorylated by Akt1 and AMPK in response to insulin and this phosphorylation is required for GLUT4 translocation in adipocytes; however, it does not affect GLUT4 internalization. Insulin-stimulated phosphorylation is significantly impaired in skeletal muscle of type 2 diabetic subjects.   Insulin treatment is also reported to increase its 14-3-3 binding capacity. AS160 phosphorylation also increases immediately after exercise and remains elevated up to 3 hours following exercise. Five isoforms of AS160 have been described that are produced by alternative splicing. Isoform 1 is highly expressed in skeletal muscle and heart and isoform 2 has higher expression in most tissues. AS160 expression is significantly reduced in pancreatic islets in type 2 diabetic subjects. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Howlett, KF., et al. (2008). Am. J. Physiol. 294(2); E401-E407; Miinea, CP., et al. (2005). Biochem. J. 391(Pt 1): 87-93; Karlsson, HKR., et al. (2005). Diabetes. 54(6); 1692-1697).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.