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Supelco

Supel™ Swift HLB DPX Tip Hamilton®

weight 3 mg (bed), volume 300 μL, box of 96 tips

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Synonym(s):
Supel™ Swift HLB DPX SPE

packaging

box of 96 tips

Quality Level

100

technique(s)

solid phase extraction (SPE): suitable

surface area

425-500 m2/g

volume

300 μL

weight

3 mg (bed)

matrix active group

Polymeric HLB phase

particle size

40-75 μm (>90%)

pore size

0.70-0.80 mL/g pore volume
80-210 Å pore size

compatibility

(MS-friendly)
for use with Hamilton

General description

DPX stands for Dispersive Pipette EXtraction. Supel™ Swift HLB DPX Tips are pipette tips that incorporate loosely contained Supel™ Swift HLB material, which is mixed with the sample solution to accomplish solid phase extraction in a dispersive manner. This DPX tip format allows for a simple and rapid workflow that is highly amenable to high throughput automation. Supel™ Swift HLB is a co-polymer solid phase extraction (SPE) phase composed of both hydrophilic and lipophilic functional groups. This phase is ideal for extracting a broad range of compounds from aqueous samples, including food/environmental samples as well as biological samples(eg. serum and plasma). Because of the hydrophilic and lipophilic balanced (HLB) property, the polymer material can selectively retain a wide range of compounds with different polarities and LogP values. Traditional HLB SPE uses a five-step process for sample preparation that normally includes the conditioning of sorbents with an organic solvent followed by an equilibration step to return sorbents to sample loading conditions. The product design allows for direct sample loading on to the sorbent bed, thereby eliminating the conditioning step altogether. This product feature can both save time and simplify sample preparation, which may also serve to reduce sample processing errors.

Application

  • Clinical
  • Bioanalysis
  • Pharmaceutical
  • Food & Bev
  • Environmental

Features and Benefits

  • Hydrophilic Modified Styrene Resin - extract and recover a broad range of analytes using a single sorbent (polar to nonpolar, acidic to basic)
  • Greater Capacity - allows for smaller bed weights, lower elution volumes, and time savings in sample processing
  • Resistant to Over-Drying - robust methodology, lower variability
  • Stringent Production & QC Standards - greater product consistency for improved accuracy and precision over all SPE formats

Analysis Note

The 96-tip dispersive SPE format is perfect for medium to high-throughput laboratories performing large numbers of samples at a time as the format is automation compatible. Recommended weight tip based on sample volume:

  • Sample Volume: <300 µL (Bed Weight: 3 mg or 5 mg)
  • Sample Volume: <600 µL (Bed Weight: 10 mg)
  • Sample Volume: <800 µL (Bed Weight: 20 mg)

Legal Information

Hamilton is a registered trademark of Hamilton Co.
Supel is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

Steroid Hormone Analysis in Serum using Supel™ Swift HLB DPX Tips

This application note describes an accurate and sensitive, Supel™ Swift HLB DPX Tips based analysis of a total of 9 steroids (cortisone, cortisol, 11-deoxycortisol, androstenedione, testosterone, dehydroepiandrosterone, 5α-dihydrotestosterone, 17α-hydroxyprogesterone, and progesterone) in blood serum samples using two different standards (UTAK and NIST).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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