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Merck
CN

577528-U

BIOshell™ A160 Peptide Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonym(s):

BIOshell™ A160 Peptide Phenyl-Hexyl, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Key Documents

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Product Name

BIOshell™ A160 Peptide Phenyl-Hexyl, 2.7 μm HPLC Column, column L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Agency

suitable for USP L11

product line

BIOshell™

feature

endcapped

packaging

1 ea of

extent of labeling

4.7% Carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

15 cm × 2.1 mm

surface area

90 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

Phenyl-Hexyl (dimethylphenyl-hexylsilane) bonding phase

particle size

2.7 μm

pore size

160 Å pore size

operating pH range

2-9

separation technique

reversed phase

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General description

BIOshell™ A160 Peptide Phenyl-Hexyl is a high-speed, high-performance liquid chromatography column based on a 160 Å Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency for high molecular weight solutes (up to 20 kDa) due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The end-capped, phenyl-hexyl bonded phase of the Peptide Phenyl-Hexyl provides a stable, reversed phase packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides, using typical acidic mobile phases favored for proteomic applications.

Application

UHPLC Analysis of Peptides on BIOshell™ A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm

Analysis Note

The target analytes include peptides and polypeptides < 20 kDa.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
USFPH001261
USFPH001260
USFPH001259
USFPH001258
USFPH001257

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Articles

A Comparison of Superficially Porous Particle-based Column Chemistries for Peptide Mapping

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

Related Content

Large Molecule HPLC

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

大分子HPLC_生物分子反相HPLC_高效液相色谱法-默克生命科学

生物分子是活细胞产生的大分子聚合物，作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择，最常用的还是高效液相色谱法(HPLC)。

Large Molecule HPLC

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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