产品名称
BIOshell™ A160 Peptide CN, 2.7 μm HPLC Column, 2.7 μm particle size
material
stainless steel hardware (column)
Quality Segment
agency
suitable for USP L10
description
Shell thickness (0.5 μm), Solid Core (1.7 μm)
product line
BIOshell™
mol wt
2000 - 20,000
feature
endcapped
analyte chemical class(es)
peptides
manufacturer/tradename
BIOshell™
packaging
1 ea of
extent of labeling
2.2% carbon loading
parameter
600 bar max. pressure, 90 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
3 cm × 2.1 mm
surface area
90 m2/g
surface coverage
2.2 μmol/m2
matrix
spherical silica particle platform, superficially porous particle
matrix active group
cyano phase
particle size
2.7 μm
pore size
160 Å
operating pH range
1-8
separation technique
hydrophilic interaction (HILIC), normal phase, reversed phase
General description
BIOshell™ 160Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C).
Legal Information
BIOshell is a trademark of Sigma-Aldrich Co. LLC
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