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About This Item
UNSPSC Code:
41115711
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
15 cm × 6 mm
Particle size:
5 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
125 Å
Matrix:
polymer particle platform
Product Name
TSKgel® Octadecyl-2PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 6 mm, 5 μm particle size
material
stainless steel hardware
product line
TSKgel®
manufacturer/tradename
Tosoh 17501
extent of labeling
1 meq/mL ligand density (estimate)
parameter
0-100% organic solvent, 1.5 mL/min flow rate, 120 bar max. pressure, 4-50 °C temperature
technique(s)
HPLC: suitable
L × I.D.
15 cm × 6 mm
matrix
polymer particle platform
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
125 Å
operating pH
2-12
separation technique
reversed phase
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General description
Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
Physical form
Shipped in 55% acetonitrile - 45% water.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Regulatory Information
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