material
polyethylene column, polypropylene and polyethylene frit
product line
TOYOPEARL®
mol wt
2000 - 1,000,000
specific analyte(s)
IgG
packaging
pkg of 1 ea
manufacturer/tradename
Tosoh 45161
parameter
3 bar max. pressure (resin), 30 bar max. pressure (column)
technique(s)
HPLC: suitable, LPLC: suitable
L × I.D.
10 cm × 8 mm
bed volume
5 mL
matrix
hydroxylated methacrylic polymer
matrix active group
rProtein A phase
particle size
45 μm
pore size
1000 Å
operating pH
2-13
capacity
>70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time), >80 g/L adsorption capacity (IgG)(static)
separation technique
affinity
General description
MiniChrom columns for TOYOPEARL® and TSKgel® are pre-packed with the most popular ion exchange, mixed-mode, hydrophobic interaction or affinity media. These columns are available in 5 mL volume, 8 mm ID x 10 cm L, and can be operated with standard laboratory LC systems. MiniChrom columns for TOYOPEARL and TSKgel provide:
- Direct connection to common laboratory LC Systems
- Easy parameter screening and method optimization
- Fast small scale purification
- Reliable and reproducible results
Features and Benefits
Clean in place with 0.1 M NaOH.
Physical form
Shipped in 20% (v/v) ethanol.
Other Notes
Columns are tolerant to aqueous buffers and salt solutions, 1 mol/L alkaline solutions, 1 mol/L non-oxidizing mineral acids, 8 mol/L urea, 6 mol/L guanidine hydrochloride, non-halogenated organic solvents and detergents. They are not compatible with strong oxidants and halogenated solvents.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation