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UV-Visible Solvents

Two amber bottles of Uvasol® isooctane for spectroscopy

Ultraviolet-visible (UV-Vis) spectroscopy is a widely used analytical technique for measuring the absorbance of light in the ultraviolet (200–400 nm) and visible (400–800 nm) regions of the electromagnetic spectrum. This method relies on the principle that different compounds absorb light at specific wavelengths, enabling their identification and quantification.

Selecting the right solvent is critical for accurate UV-Vis analysis. Ideal solvents have high transparency in the measured wavelength range, minimizing background absorption that could interfere with analyte detection.


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Uvasol® Solvents

Uvasol® solvents are high-purity, optically transparent solvents designed for UV-Vis and infrared (IR) spectroscopy. With the highest UV transmittance and lowest UV absorbance, they ensure precise and reliable analytical results while minimizing the risk of misinterpretation.

These solvents comply with Ph. Eur. (European Pharmacopoeia) and USP (United States Pharmacopeia) methods, meeting stringent UV transmittance and absorbance standards as defined by Reag. Ph Eur and ACS specifications. Their exceptional batch-to-batch consistency reduces the need for repeat analyses, saving both time and costs. Additionally, Uvasol® solvents eliminate interferences from UV, IR, and fluorescence contamination, further enhancing analytical accuracy and application security.

Beyond UV-Vis applications, Uvasol® solvents are also suitable for IR spectroscopy. Commonly used solvents in this range include acetone, acetonitrile, 1-butanol, 2-propanol, chloroform, cyclohexane, dichloromethane, dimethyl sulfoxide (DMSO), ethanol, methanol, ethyl acetate, and n-heptane.

Acetone

Acetone is a polar aprotic solvent known for its strong solvating power, making it effective in dissolving a wide range of organic and inorganic compounds. It is highly transparent in the UV-Visible spectrum, with minimal absorbance above 330 nm, ensuring accurate spectroscopic measurement for analytes that absorb at lower wavelengths.

Acetonitrile

Acetonitrile is a highly transparent polar aprotic solvent, making it ideal for UV-Visible spectroscopy. With minimal absorbance below 210 nm, it allows precise measurement of analytes in the lower UV range. Its excellent solvating properties and compatibility with a wide range of reagents make it a preferred choice for chromatography, spectroscopic analyses, and other analytical applications.

Cyclohexane

Cyclohexane is a non-polar solvent commonly used for analyzing hydrophobic substances. It exhibits excellent transparency in the UV-Visible range, with minimal absorbance below 200 nm, making it suitable for detecting analytes that absorb in the deep UV region. Its low reactivity and high purity further enhance its suitability for spectroscopic and chromatographic applications.

Dimethyl Sulfoxide (DMSO)

Dimethyl sulfoxide (DMSO) is a highly polar aprotic solvent known for its strong solvating power and excellent transparency in the UV-Visible range. It exhibits minimal absorbance below 300 nm, making it suitable for spectroscopic analyses that require low background interference. 

Ethanol

Ethanol is a polar solvent with excellent transparency in the UV-Visible range, exhibiting minimal absorbance above 210 nm. Its compatibility with a wide range of biological molecules makes it a preferred solvent in biochemical assays, spectroscopic analyses, and pharmaceutical applications. Ethanol’s low UV absorbance ensures accurate measurement of analytes, particularly in biological and pharmaceutical research.

Isooctane

Isooctane is a highly non-polar solvent with exceptional transparency in the UV-Visible range, exhibiting very high transmittance even below 200 nm. Its low absorbance at deep UV wavelengths makes it an excellent choice for spectroscopic analyses, ensuring accurate and reliable analytical results. Isooctane is commonly used in UV-Vis spectroscopy and chromatographic applications where minimal solvent interference is critical.

Tetrahydrofuran (THF)

Tetrahydrofuran (THF) is a polar aprotic solvent with excellent transparency in the UV-Visible range, exhibiting low absorbance below 240 nm. This property makes it ideal for spectroscopic analyses, allowing accurate measurement of analytes in the UV region with minimal solvent interference. THF’s low viscosity enhances its handling and promotes efficient mixing with solutes, ensuring uniform and reliable solutions for analytical applications.



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