Advanced gene editing

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Zinc Finger Nuclease References: Human, Rat, Mouse, Zebrafish, Plasmodium, Xenopus, Rainbow Trout, Mosquito, Pig, Rabbit, Cattle, CHO, ZFN Technology, Reviews, Posters, Label License

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Oligonucleotide Quality Control by Mass Spectrometry

Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.

Oligonucleotide Quality Control by Analytical HPLC

In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

ThermaGenix Product Overview

How to stop nonspecific bands and off target primers in PCR.

Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

General Considerations for Plasmid DNA Preparation

This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.

RNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during RNA preparation.

Genomic DNA Preparation Troubleshooting

Description of common problems that can occur during genomic DNA preparation

A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®

Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.

DNA and RNA Quantification

An overview of methods for quantifying DNA and RNA, how to measure DNA and RNA concentration and yield, and how to assess purity of nucleic acid samples.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Colony PCR

Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Dual-Labeled Probes in Digital PCR

Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.

Custom LAMP Primers in Partnership with New England Biolabs

Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.

KAPA Long Range PCR Kits FAQs

Review some of the most asked questions regarding the KAPA Long Range PCR system, which is a blend of Taq DNA polymerase and an engineered archaeal DNA polymerase with proofreading capability.

KAPA2G Robust PCR Kits FAQs

Review some of the most asked questions regarding the KAPA2G Robust PCR Kits, which consists of KAPA2G Robust DNA Polymerase to address inconsistent amplification and KAPA2G Robust ReadyMix.

Roche PCR Reagents and PCR Protocols

Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.

Small Molecule CRISPR Enhancers

Modulation of homology-directed repair (HDR) within the context of CRISPR-genome editing has led to the identification of small molecules that enhance CRISPR-mediated HDR efficiency in various cell types.

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

RT-PCR Troubleshooting

PCR troubleshooting guide for reliable data in molecular assays.

KAPA2G Fast PCR Kits FAQ

Fast PCR reduces reaction time, enhancing efficiency with optimized cycling parameters.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

PCR/qPCR/dPCR Assay Design

PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.

Cloning Troubleshooting

Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.

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