Gene expression and silencing

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facet applications:Gene expression and silencing

facet applications:Transfection and gene editing

facet content type:Protocol

Showing 1-30 of 41 results

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Simplicon™ RNA Transfection and Electroporation Protocols

Biodegradable Nanoparticles

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Antibiotic Optimization for Cell Transduction

Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

hESC Transduction Protocol

A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.

Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix

MystiCq® MicroRNA® Quantitation System

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

MISSION® shRNA Guarantee

Guarantee efficient gene knockdown with our shRNA clones, ensuring reliable results for targeted transcript suppression.

Spinoculation Protocol

Protocol for lentiviral transduction of suspension cells using MISSION TRC shRNA Lentiviral Particles for long-term silencing.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Liposome Preparation - Avanti® Polar Lipids

Lipid vesicle preparation involves hydration and sizing steps, applicable regardless of lipid composition.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti^® Polar Lipids

This procedure describes the preparation of cationic liposomes and transfection of cells.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

磷酸钙转染试剂盒实验方案

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

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