Mammalian cell culture

Applied Filters:

facet applications:Mammalian cell culture

facet content type:Technical Article

Showing 1-30 of 117 results

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

F-12 Coon's Modification Formulation

L-15 Media Formulation

Iscove's Modified Dulbecco's Media (IMDM) Formulation

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

Cell Freezing and Banking

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

VEGF Pathway

Read review on VEGF signaling pathway (Vascular endothelial growth factor (VEGF)/VEGF receptor) and find related products.

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342.

Autophagy in Cancer Promotes Therapeutic Resistance

We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

NF-κB and Inflammation

Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.

Alternative High-Yield Cell Culture Systems

Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Identification of Cell Culture Media with Raman Spectroscopy

Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Linolenic Acid in Cell Culture

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Media Formulations

Compare all of the formulation tables for cell culture in one place.

L-Ornithine in Cell Culture

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

L-Tyrosine in Cell Culture

Learn more about the amino acid L-Tyrosine and explore how it is used in cell culture media like MEM, DMEM, and RPMI. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

DMEM/nutrient Mixture F-12 Formulation

Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation

Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems

Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.

Glucose in Cell Culture

Importance and uses of glucose in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Ferric and Ferrous Iron in Cell Culture

Importance and uses of ferric and ferrous iron in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Biotin in Cell Culture

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Page 1 of 4