Nucleic acid labeling and detection

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facet applications:Nucleic acid labeling and detection

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ABI3900 Synthesizer Columns with CUTAG CPG Frits

Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

NBT Protocol & Troubleshooting

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

DIG Northern Starter Kit Protocol & Troubleshooting

DNA probes, like PCR or random-primed DNA probes, are effective for northern blots, particularly for detecting abundant messages.

Oligonucleotide Melting Temperature

Learn about methods for calculating oligonucleotide melting temperature (Tm).

NBT/BCIP Stock Solution Protocol & Troubleshooting

NBT Protocol

DIG Easy Hyb™ Granules Protocol & Troubleshooting

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

DIG RNA Labeling Mix Protocol Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Dextran Sulfate

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.