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Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
ThermaGenix Product Overview
How to stop nonspecific bands and off target primers in PCR.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
PCR Troubleshooting Tips
When developing a PCR troubleshooting protocol, it is important to be open to any possible sources of error, however insignificant they may seem, in order to explore each potential problem independently.
RT-PCR / RT-qPCR Troubleshooting
Developing a PCR or RT-PCR/RT-qPCR troubleshooting protocol so that data are reliable is essential. Potential sources of RT-PCR or PCR error and problems include operator error, the PCR master mix, and oligo design. This PCR troubleshooting guide outlines and details
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
PCR Master Mix
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
5'/3' RACE Kit, 2nd Generation Troubleshooting
In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
MIQE – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Our qPCR services, including primer and probe designs, assay protocol development, troubleshooting, and data analysis support, adhere to MIQE, which allows you to publish or bring your product to market faster and with confidence.
PCR/qPCR/dPCR Assay Design
The entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and
Colony PCR
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Extract-N-Amp™ Product Guide
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Troubleshooting for Molecular Cloning
Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein
Dual-Labeled Probes in Digital PCR
Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
Custom LAMP Primers in Partnership with New England Biolabs
Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
KAPA Long Range PCR Kits FAQs
Review some of the most asked questions regarding the KAPA Long Range PCR system, which is a blend of Taq DNA polymerase and an engineered archaeal DNA polymerase with proofreading capability.
Whole Genome Amplification (WGA) for Single Cell Biology
Advances in single-cell WGA have enabled the contribution of genomics to single-cell biology. Whole-genome amplification (WGA) is described as a non-specific amplification that affords a product completely representative of the initial starting material.
Review some of the most asked questions regarding the KAPA2G Fast PCR Kits, which consists of a second-generation enzyme for high yields and sensitivity across a broad range of targets.
KAPA2G Robust PCR Kits FAQs
Review some of the most asked questions regarding the KAPA2G Robust PCR Kits, which consists of KAPA2G Robust DNA Polymerase to address inconsistent amplification and KAPA2G Robust ReadyMix.
Roche PCR Reagents and PCR Protocols
Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.
Mycoplasma PCR ELISA Protocol
Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma
Locked Nucleic Acid
Frequently asked questions about Locked Nucleic Acids (LNA)
Custom cGMP Oligos
If you commercialize Molecular Diagnostics (MDx), develop Laboratory Developed Tests (LDTs), or conduct preclinical research on therapeutics, then your final kit, assay, or modality must have an extraordinary level of quality to comply with strict regulatory or study standards.
Water for PCR Techniques
Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.
FastStart™ Taq DNA Polymerase, dNTPack Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA
The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis
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