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Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
ThermaGenix Product Overview
How to stop nonspecific bands and off target primers in PCR.
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
5'/3' RACE Kit, 2nd Generation Troubleshooting
In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
Colony PCR
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Extract-N-Amp™ Product Guide
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Dual-Labeled Probes in Digital PCR
Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
Custom LAMP Primers in Partnership with New England Biolabs
Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.
KAPA Long Range PCR Kits FAQs
Review some of the most asked questions regarding the KAPA Long Range PCR system, which is a blend of Taq DNA polymerase and an engineered archaeal DNA polymerase with proofreading capability.
KAPA2G Robust PCR Kits FAQs
Review some of the most asked questions regarding the KAPA2G Robust PCR Kits, which consists of KAPA2G Robust DNA Polymerase to address inconsistent amplification and KAPA2G Robust ReadyMix.
Roche PCR Reagents and PCR Protocols
Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.
RT-PCR Troubleshooting
PCR troubleshooting guide for reliable data in molecular assays.
KAPA2G Fast PCR Kits FAQ
Fast PCR reduces reaction time, enhancing efficiency with optimized cycling parameters.
PCR Master Mix
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
PCR/qPCR/dPCR Assay Design
PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.
Cloning Troubleshooting
Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.
MIQE: Real-Time PCR Experiment Standards
MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.
PCR Troubleshooting Tips
PCR troubleshooting protocols require exploring all potential error sources to ensure reliable results, addressing each problem independently.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher
Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.
Water for PCR Techniques
Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Water for Next-Generation Sequencing
Explore the essential role of water in next-generation sequencing for accurate and efficient genomic analysis
Custom cGMP Oligos
If you commercialize Molecular Diagnostics (MDx), develop Laboratory Developed Tests (LDTs), or conduct preclinical research on therapeutics, then your final kit, assay, or modality must have an extraordinary level of quality to comply with strict regulatory or study standards.
Polymerase Chain Reaction
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
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