Primary cell culture

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Protocol for Preparation and Cultivation of PromoCell® Cryopreserved and Proliferating Cells

Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Primary Cell Culture FAQ

Primary Cell Culture: Frequently Asked Questions (FAQs)

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Lung Fibroblasts (HLF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Epidermal Keratinocytes (HEK) Culture Protocol

Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

Rat Skeletal Muscle Cells (RSkMC) Culture Protocol

Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation

Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol

Technical information for working with rat brain microvascular endothelial cells including thawing, subculturing and cryopreservation

Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Primary Human Hepatocytes Culture Protocol

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

Human Epidermal Melanocytes (HEM) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Culturing Human Aortic Smooth Muscle Cells

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Human Chondrocytes (HC) Culture Protocol

Cryovial handling precautions prevent injuries from liquid nitrogen bursts.

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

Primary Human Hepatic Kupffer Cell Culture Protocol

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

Protocol Guide: Motor Neuron Monoculture

Discover an expert protocol for seeding and culturing motor neurons in monoculture. Explore high purity motor neurons culture cells and reagents.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Human Bronchial Epithelial Cell line

Technical information for working with human bronchial epithelial cell line including thawing, subculturing and cryopreservation.

Human Dermal Fibroblasts (HDF) Culture Protocol

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

Human Follicle Dermal Papilla Cells (HFDPC) Culture Protocol

Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.

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