Transfection and gene editing

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Detect, Visualize and Quantify Single Post-Translational Modifications

Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications

Functionalized PEI and Its Role in Gene Therapy

Gene therapy has become one of the most discussed techniques in biomedical research in recent years.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

siRNA Design, Manufacturing, Transfection & Detection

RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.

Lentiviral Transduction

In this exercise we will be demonstrating lentiviral transduction on two different cell lines.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Liposome Preparation - Avanti® Polar Lipids

Lipid vesicle preparation involves hydration and sizing steps, applicable regardless of lipid composition.

Transfection & Gene Editing

Cell transfection principles, common transfection methods, and the role of transfection in gene editing and silencing for research, drug discovery, recombinant protein production.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the

Simplicon™ RNA Transfection and Electroporation Protocols

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications

Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.

siRNA Delivery in Mammalian Cell Lines

siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.

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