1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting

Tris-buffered saline (TBS) is an excellent wash buffer for many types of immunoassays, such as ELISAs, immunohistochemisty, and Western blots. To make 1 L of TBS wash buffer, add 100 mL of 10X TBS to 900 mL of water.

This calculator enables the accurate preparation of a 1X TBS working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.

1X Solution concentrations:

• Tris: 20 mM
• NaCl: 150 mM

TBS Recipe Calculator

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS AND SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• 10X Tris-Buffered Saline (TBS) Stock Solution
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X Phosphate-Buffered Saline (PBS)
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Non-Fat Dry Milk (NFDM) Blocking Buffer
• Bovine Serum Albumin (BSA) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions