- LC-MS/MS studies of ritonavir and its forced degradation products.
LC-MS/MS studies of ritonavir and its forced degradation products.
Journal of pharmaceutical and biomedical analysis (2010-07-14)
R Nageswara Rao, B Ramachandra, R Mastan Vali, S Satyanarayana Raju
PMID20619992
ABSTRACT
Forced degradation of ritonavir (RTV), under the conditions of hydrolysis (acidic, basic and neutral), oxidation, photolysis and thermal stress as prescribed by ICH was studied using LC-MS/MS. Eight degradation products were formed and their separation was accomplished on Waters XTerra C(18) column (250 mm x 4.6 mm i.d., 5 microm) using water:methanol:acetonitrile as (40:20:40, v/v/v) mobile phase in an isocratic elution mode by LC. The method was extended to LC-MS/MS for characterization of the degradation products and the pathways of decomposition were proposed. No previous reports were found in the literature regarding the characterization of degradation products of ritonavir.
MATERIALS
Product Number
Brand
Product Description
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm, pkg of 2 ea
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 3 mm
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm, pkg of 2 ea