Scalability of Separations on SeQuant® ZIC-HILIC. Analytical to Semi-Preparative Scale
CONDITIONS
column
SeQuant ZIC-HILIC, PEEK, 50, x 2.1 mm, 5 μm, 200 Å (<href="/ProductLookup.html?ProdNo=150450&Brand=MM">1.50450.0001,<href="/ProductLookup.html?ProdNo=150451&Brand=MM">1.50451.0001 &Brand=MM">1.50451.0001)
mobile phase
[A] acetonitrile; [B] 5 mM ammonium acetate; (80:20, A:B)
flow rate
0.1, 0.5, 1.3, 9.3 mL/min
pressure
144, 144, 115, 158 psi (1.0, 1.0, 0.8, 1.1 MPa)
detector
254 nm
injection
1, 5, 20, 100 μL
Description
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany