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column	Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 µm particles (59602-U)
column temp.	25 °C
mobile phase	([A] 20 mM Tris, pH 8.0 with hydrocloric acid; [B] 20 mM Tris, 0.5 M sodium chloride, pH 8.0 with hydrochloric acid)
gradient	0 to 10% B in 30 min. (linear)
flow rate	0.5 mL/min
injection	10 µL
detector	UV, 280 nm

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Analysis Note	Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
Categories	Analytical Chromatography, Proteins
Featured Industry	Life Science and Biopharma
suitability	application for HPLC

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