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column	SeQuant^® ZIC-cHILIC, PEEK, 250 x 4.6 mm, 3 μm, 100Ã… (1.50662.0001)
column temp.	45 °C (oven), 40 °C (detector cell)
mobile phase	[A] acetonitrile; [B] 0.1% ortho-phosphoric acid in Milli-Q^® water; (90:10, A:B)
flow rate	1.5 mL/min
pressure	1682 psi (116 bar)
sample	5.0 mg/mL (5000 ppm) each of sorbitol and mannitol in diluent. (Diluent: Acetonitrile: 0.1% ortho-phosphoric acid in Milli-Q^® water 75:25 (v/v))
injection	20 μL
detector	Refractive Index (RI) (cell: 10 μL)

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Featured Industry	Pharmaceutical (small molecule)
suitability	application for HPLC

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LC/MS Analysis of Sorbitol and Mannitol on SeQuant® ZIC-HILIC

Materials

LC/MS Analysis of Sorbitol and Mannitol on SeQuant^® ZIC-HILIC