{"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"keywords":["Solid phase extraction","Protocol","Mass spectrometry","Gas chromatography"],"title":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB®-5ms after SPE using Discovery DSC-Si","seoSchemas":[{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Ion Exchange Methodology","item":"%%HOMEPAGE_URL%%/CN/en/technical-documents/technical-article/analytical-chemistry/solid-phase-extraction/ion-exchange-methodology"},{"@type":"ListItem","position":2,"name":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB^®-5ms after SPE using Discovery DSC-Si"}]}],"description":"Separation of Cholesterol; Brassicasterol; Campesterol; Stigmasterol; β-Sitosterol","pageType":"Protocol"},"title":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB®-5ms after SPE using Discovery DSC-Si","lastModifiedDate":1600949140864,"templateName":"technical-article---protocol-page-template","designPath":"/libs/settings/wcm/designs/default","cssClassNames":"page basicpage","language":"en",":hierarchyType":"page",":itemsOrder":["root"],":items":{"root":{"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"columnCount":12,"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","allowedComponents":{"applicable":false,"components":[{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/recommendation","title":"Recommendation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1600949140696,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/topresponsivecontainer/tagbasedbreadcrumb","name":"tagbasedbreadcrumb"},{"url":null,"title":null,"description":null,"lastModified":1600949140700,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/topresponsivecontainer/title","name":"title"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["tagbasedbreadcrumb","title"],":items":{"tagbasedbreadcrumb":{"items":[{"url":"/CN/en","title":"Home","active":false,"nonClickable":false},{"url":"/CN/en/applications/analytical-chemistry/sample-preparation/solid-phase-extraction","title":"Solid Phase Extraction (SPE)","active":false,"nonClickable":false},{"url":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816","title":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB^®-5ms after SPE using Discovery DSC-Si","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"openNewTab":false,"linkURL":null,"text":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB^®-5ms after SPE using Discovery DSC-Si","type":"h1",":type":"cms-commons/components/content/title"}},":type":"cms-commons/components/content/container"},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},":type":"cms-commons/components/content/container"},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1600949140759,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer","name":"topcontainer"},{"url":null,"title":null,"description":null,"lastModified":1600949140829,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/dynamicproducttable","name":"dynamicproducttable"},{"url":null,"title":null,"description":null,"lastModified":1600949140834,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/middleresponsivecontainer","name":"middleresponsivecontainer"},{"url":null,"title":null,"description":null,"lastModified":1600949140838,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/reference","name":"reference"},{"url":null,"title":null,"description":null,"lastModified":1600949140841,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/bottomcontainer","name":"bottomcontainer"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"],":items":{"topcontainer":{"columnClassNames":{"image":"aem-GridColumn aem-GridColumn--default--8 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","text_copy":"aem-GridColumn aem-GridColumn--default--12","table_1554176040":"aem-GridColumn aem-GridColumn--default--12","text":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12","text_99016783":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1600949140791,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer/image","name":"image"},{"url":null,"title":null,"description":null,"lastModified":1600949140804,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer/text","name":"text"},{"url":null,"title":null,"description":null,"lastModified":1600949140809,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer/table","name":"table"},{"url":null,"title":null,"description":null,"lastModified":1600949140814,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer/text_copy","name":"text_copy"},{"url":null,"title":null,"description":null,"lastModified":1600949140819,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer/table_1554176040","name":"table_1554176040"},{"url":null,"title":null,"description":null,"lastModified":1600949140824,"path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816/jcr:content/root/bodyresponsivecontainer/topcontainer/text_99016783","name":"text_99016783"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["image","text","table","text_copy","table_1554176040","text_99016783"],":items":{"image":{"anchorId":null,"externalUrl":false,"widths":[],"alt":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil","title":"GC Analysis of Sterols (Silylated Derivatives) in Olive Oil","uuid":"0936a45d-dc7d-449b-a6f7-082fc26bcfb3","src":"/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols/gc-analysis-of-sterols.jpg","link":"","caption":"","displayPopupTitle":true,":type":"cms-commons/components/content/image"},"text":{"text":"

Conditions

\r\n","richText":true,":type":"cms-commons/components/content/text"},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":null,"title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

sample preparation	SPE (Solid Phase Extraction)
sample/matrix	olive oil spiked with a mixed sterol standard at 5 µg/g
SPE tube/cartridge	Discovery DSC-Si, 1 g/6 mL (52656-U)
condition	2 x 5 mL hexane
sample addition	1 mL
elution	esters fraction: 2.5 mL of hexane:ethyl acetate (90:10); free sterols fraction; 5 mL of hexane:ethyl acetate (90:10) and 3 mL x 5 of ethanol:diethyl ether:hexane (50:25:25)
eluate post-treatment	Evaporate to dryness and perform silylation as described in the Analysis Note.
column	SLB-5ms, 20 m x 0.20 mM I.D., 0.20 µm (28564-U)
oven	125 °C (1 min), 10 °C/min to 325 °C (10 min)
carrier gas	helium, 0.6 mL/min, constant
sample	Olive oil sample; fractionated, transesterified, and silylated
injection	1 µL, splitless
liner	4 mM I.D. single taper
inj. temp.	250 °C
detector	MSD, rn/z 500 - 600
MSD interface	325 °C

\r\n",":type":"cms-commons/components/content/table"},"text_copy":{"text":"

Description

\r\n","richText":true,":type":"cms-commons/components/content/text"},"table_1554176040":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":null,"title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Analysis Note	Sterol content is one in a battery of tests used to analyze the composition and determine the grade and authenticity of olive oil. Test results are compared against standards such as those established by the International Olive Oil Council (IOC) and US Dept. of Agriculture. In vegetable oils, sterols exist in the free form, or esterified to fatty acids. In this application, sterols were analyzed in olive oil separately as free and esterified. This was accomplished by taking advantage of the polarity difference between these two groups to fractionate them using silica gel SPE. In place of saponification, the fractions were transesterified to liberate the esterified sterols. The fractions were then silylated, and analyzed by GC-MS. The silylated sterols all contained a significant molecular ion in their mass spectum, which made it possible to perform peak identification free of matrix interference. The sterols isolated in the free fraction are shown here., Sample pre-treatment \r\nWeigh 0.25 gm oil into test tube. For spiked sample add 12.5 μL of 65-100 μg/mL mixed sterol std. Add 1 ml of hexane:ethyl acetate (90:10). After SPE fractionation, evaporate solvent from each fraction, add 1 ml of 30% methanolic sodium methoxide:MeOH:MTBE solution (13:27:60), mix and let sit at room temp for 30 min. Add 1 ml of water and 2 mL of n-heptane. Withdraw aqueous phase (bottom) and replace w/ 1 mL of 1% citric acid solution. Withdraw organic phase and dry over anhydrous sodium sulfate. Silylation: Evaporate organic phase to dryness. Add 125 μL of Sylon BFT and 125 μL of pyridine. Heat at 70°C for 20 min. The sample is then ready for GC-MS analysis.
Categories	Analytical Chromatography, SPE Applications, GC Applications, Sterols
Featured Industry	Agriculture \r\nFood and Beverages
Other Notes	Complementary products: \r\nSyringes \r\nVials \r\nGas Purification and Gas Management \r\nInlet Liners, Glass Wool, and Specialized Hand Tools \r\nSepta and Specialized Hand Tools \r\nColumn Ferrules, Nuts, and Specialized Hand Tools
suitability	application for GC, application for SPE

\r\n",":type":"cms-commons/components/content/table"},"text_99016783":{"text":"

Analyte

\r\n

Brassicasterol
Campesterol
Cholesterol
Stigmasterol
β-Sitosterol

\r\n","richText":true,":type":"cms-commons/components/content/text"}},":type":"cms-commons/components/content/container"},"dynamicproducttable":{"anchorId":null,"productsArray":["Y0001615 Sigma-Aldrich (SIAL)","B4936 Sigma-Aldrich (SIGMA)","C5157 Sigma-Aldrich (SIGMA)","C2155000 Sigma-Aldrich (SIAL)","PHR1533 Supelco (SIAL)","47127-U Supelco (SUPELCO)","52656-U Supelco (SUPELCO)","43623 Supelco (SIAL)","47133 Supelco (SUPELCO)","28564-U Supelco (SUPELCO)","S2424 Sigma-Aldrich (SIGMA)","47132 Supelco (SUPELCO)"],"isShowImage":false,"maxRows":20,"products":[{"productKey":"Y0001615","brandKey":"SIAL"},{"productKey":"B4936","brandKey":"SIGMA"},{"productKey":"C5157","brandKey":"SIGMA"},{"productKey":"C2155000","brandKey":"SIAL"},{"productKey":"PHR1533","brandKey":"SIAL"},{"productKey":"47127U","brandKey":"SUPELCO"},{"productKey":"52656U","brandKey":"SUPELCO"},{"productKey":"43623","brandKey":"SIAL"},{"productKey":"47133","brandKey":"SUPELCO"},{"productKey":"28564U","brandKey":"SUPELCO"},{"productKey":"S2424","brandKey":"SIGMA"},{"productKey":"47132","brandKey":"SUPELCO"}],"tableTitle":"Materials",":type":"cms-commons/components/content/dynamicproducttable"},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},":type":"cms-commons/components/content/container"},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},":type":"cms-commons/components/content/container"}},":type":"cms-commons/components/content/container"},"rightrailresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},":type":"cms-commons/components/content/container"},"footer":{"richText":false,":type":"cms-commons/components/content/footer"}},":type":"wcm/foundation/components/responsivegrid"}},":type":"cms-commons/components/structure/page",":path":"/CN/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-sterols-silylated-derivatives-in-olive-oil-free-sterol-fraction-g005816"}