Salmonella Detection in the Food Chain (EN ISO 6579-1)
The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food production chain. Like the preceding ISO 6579:2002/Amd 1:2007, the latest ISO 6579-1:2017 covers the testing of products intended for human consumption, animal feed, and environmental samples in food production and handling. In addition, EN ISO 6579-1:2017 now includes testing of milk and milk products as well as samples from the primary production stage, such as animal feces, dust and boot socks.
EN ISO 6579-1 forms part one of the three-part EN ISO 6579, titled “Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella”. The complementary EN ISO/TS 6579-2, which outlines a procedure for Salmonella enumeration, and EN ISO/TR 6579-3, which provides guidance for the serotyping of Salmonella, have already been published.
Procedure for Salmonella detection according to EN ISO 6579-1
Day 2 - Selective enrichment
| 0.1 mL BPW to 10 mL RVS broth or MSRV agar 24 h (± 3) • 41.5 °C (± 1) |
1 mL BPW to 10 mL MKTTn 24 h (± 3) • 37 °C (± 1) |
Day 3 - Plating out
XLD agar 24 h (± 3) • 37 °C (± 1) + Second isolation agar
Day 4, 5, and 6 – Confirmation and Expression of result
Test at least 1 typical or suspect colony.
If negative, test additional 4 marked colonies.
Non-selective agar (e.g. Nutrient Agar)
24 h (± 3) • 34 °C - 38 °C
Biochemical/serological testing Expression of results
EN ISO 6579-1 at a Glance
- Part one (detection) of three-part EN ISO 6579 replaces EN ISO 6579:2004+Amd 1:2007
- EN ISO 6785:2007 (dairy products) method details now incorporated
- Samples from primary production stage added to scope
- Incubation temperature range for non-selective media extended to 34 – 38 °C
- Choice of RVS or MSRV agar for selective enrichment of food, animal feed and environmental samples
- Directions for choice of second isolation medium in addition to XLD agar
- Only one suspect colony needed for confirmation (if negative, 4 more to be tested)
- Optional: biochemical confirmation on well-isolated colony direct from selective plate
- β-galactosidase and indole tests now optional, Voges-Proskauer reaction omitted
- Detection of Salmonella Typhi and S. Paratyphi described
- Performance testing for the culture media in-depth
EN ISO 6579-1 Compliant Culture Media
As a worldwide leading provider of a broad range of dehydrated granulated and ready-to-use culture media for food, beverages, and water microbiology, we closely track and support the development of relevant standards aimed at increasing consumer confidence and safety.
Compliance to EN ISO 6579-1
Our GranuCult® granulated media, Readybag® pre-weighed granulated media in ready-to-use bags, ReadyStream®, EcoCult powder media, ReadyPlate™ & ReadyTube® ready-to-use media are all compliant with the newest version of EN ISO 11133. All information regarding compliance with additional reference standards is displayed on the product label and in the product’s technical information available on our website.
We have implemented all the requirements described in EN ISO 6579-1, and the compliant culture media and accessories listed in the table below are available for your needs.
Reagents
Culture media and Broth
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