Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing Agilent workflows. The following modifications are required:
- The TransPlex WTA amplification product is double-stranded cDNA. Labeling, hybridization, and wash procedures are preformed using the workflow outlined in the Agilent Oligonucleotide Array-Based CGH for Genomic DNA
Analysis ULS Labeling for Blood, Cells, Tissues or FFPE (with a High Throughput option) (Version 3.4, July, 2012).
- Fragmentation of the amplification product is not required.
- The Agilent Genomic DNA ULS™ (Universal Linkage System) Labeling Kit procedure (Agilent Cat. No. 5190-0419), allows for direct labelling of the microarray target, preferable to enzymatic incorporation of modified nucleotides during or following amplification.