Process Development and Intensification for Ultrafiltration/Diafiltration of Viral Vectors
WEBINAR
Fast and efficient processes with reliable scale-up capabilities are essential for viral vector manufacturing. The primary tangential flow filtration (TFF) — step-through ultrafiltration and diafiltration (UF/DF) — can intensify the purification process prior to chromatography steps.
We will explore two operating strategies, transmembrane pressure (TMP) and permeate control, while considering their impact on process time, viral yield, and impurity removal. We will also compare the performance of commercially available single-use TFF modules for processing viral vectors. These insights offer a foundation for process development and optimization of processing viral vectors.
In this webinar, you will learn about:
- The methods to develop, optimize and scale up a TFF process for viral vector manufacturing with different operating strategies
- Intensifying the downstream purification process for viral vectors
- Process performance characteristics of different single-use technologies available for viral vector manufacturing
Speakers
Anand Alembath
Merck
Development Engineer
Anand Alembath is a development engineer and the applications technical lead for tangential flow filtration (TFF) product development projects with a focus on downstream purification of viral vectors. He holds an M.S. and Ph.D. in chemical engineering from Missouri University of Science and Technology, Rolla.
Rosario Cervellere
Merck
Applications Engineer
M. Rosario Cervellere, Ph.D., is a Downstream Processing R&D Applications Engineer III, leading application work for product and technology development in both filtration and chromatography. He has contributed to the successful product launches of the Pellicon Capsule®, Millipore Express® SPG 0.2 μm vent filters, and the recently introduced Millipore Express® Ace 0.2 μm membrane. His current focus is on driving transformation through digital innovation in downstream processing, both internally and in collaboration with academic institutions and industry consortia.
Pharma and biopharma manufacturing
- Viral Vector Upstream Processing
期间:1h
语言:English
场次 1:往期 June 29, 2023