03353575910
Roche
DIG寡核苷酸3′-末端标记试剂盒，第2代
sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide), storage condition avoid repeated freeze/thaw cycles
usage
sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide)
manufacturer/tradename
Roche
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General description
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。 DIG系统是灵敏且具有成本效益的方案，可替代放射性标记和放射法检测核酸。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。
第二代寡核苷酸3′末端地高辛标记试剂盒使用了末端转移酶，可将单个地高辛标记双脱氧尿苷三磷酸(ddUTP)催化添加到寡核苷酸的3′-OH端上，从而标记寡核苷酸。
Application
第二代寡核苷酸3′末端地高辛标记试剂盒采用DIG-11-ddUTP和重组末端转移酶对14-100bp的寡核苷酸进行3′-末端标记。
DIG标记寡核苷酸已用于多种杂交技术：
DIG标记寡核苷酸已用于多种杂交技术：
- 斑点/狭缝印迹
- 菌落/斑块杂交
- Southern印迹/Northern印迹
- 原位杂交
Biochem/physiol Actions
利用重组末端转移酶将一个DIG-ddUTP分子添加到寡核苷酸的3′-末端。这能够确保获得具有高特异性和独特性的杂交信号，该信号可利用酶联免疫测定结合抗DIG-AP抗体偶联物以及一种有色或化学发光反应进行检测。
Features and Benefits
- 由于寡核苷酸具有小分子量，可用于快速杂交动力学
- 单链探针，在杂交过程中不会复性
- 可以根据实验设计序列
- 特别适用于原位杂交；由于寡核苷酸具有小分子量，因此易于扩散到固定的组织和细胞中
Packaging
1个试剂盒包含9个组分。
Preparation Note
-15–-25 °C储存。（未开封试剂盒）
活化剂：硫酸钠，Tris
工作浓度：寡核苷酸：100 pmol
在一次标准标记反应中，最多能够标记100pmol（一个30聚体为1 μg）寡核苷酸。
工作浓度：寡核苷酸：100 pmol
在一次标准标记反应中，最多能够标记100pmol（一个30聚体为1 μg）寡核苷酸。
测定时间：从标记寡核苷酸到杂交和检测到第一个可见信号的完整过程，可在24小时内完成。
样品材料
长度为14至100个核苷酸的寡核苷酸，经过HPLC或凝胶电泳纯化
样品材料
长度为14至100个核苷酸的寡核苷酸，经过HPLC或凝胶电泳纯化
Analysis Note
已通过斑点杂交试验测试功能。
Other Notes
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- Reaction Buffer 5x concentrated
- CoCl<sub>2</sub> Solution 25 mM
- DIG-ddUTP Solution 1 mM
- Recombinant Terminal Transferase 400 U/μl
- Control Oligonucleotide, unlabeled 20 pmol/μl
- Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl
- Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled
- Glycogen Solution 20 mg/ml
- DNA Dilution Buffer, 50 μg/ml fish sperm DNA
查看所有结果 (9)
Hazard Classifications
Acute Tox. 4 - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B Inhalation
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
signalword
Danger
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
低风险生物材料
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商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 3353575910 | 04061826739761 |
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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