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Merck
CN

88417

Sigma-Aldrich

MTT 甲臜

MTT reduction end product, powder

别名:

1-(4,5-二甲基吡啶-2-基)-3,5-二苯基甲酰胺, 噻唑基蓝甲臜

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About This Item

经验公式(希尔记法):
C18H17N5S
CAS号:
分子量:
335.43
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

产品名称

MTT 甲臜, MTT reduction end product

质量水平

表单

powder

溶解性

chloroform: 10 mg/mL, clear, dark violet

SMILES字符串

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

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应用

MTT甲臜用于细胞活率检测。

生化/生理作用

MTT法是一种比色反应分析方法,常用于检测细胞活率。该方法要求将溶于水的黄色3-[4,5-二甲基噻唑-2-yl]-2,5-基四氮唑嗅盐(MTT)酶促还原为不溶于水的紫色MTT-甲臜。该反应由线粒体脱氢酶催化,仅在活细胞中发生。因此,蓝色甲臜量与活细胞数量成正比。通过分光光度法分析570 nm波长处的甲臜溶液吸光度。

其他说明

评审:四唑盐和甲瓒

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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