所有图片(1)
About This Item
线性分子式:
CH3COOP(O)(OK)(OLi)
CAS号:
分子量:
184.06
Beilstein:
5689718
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.32
推荐产品
产品名称
乙酰磷酸钾锂, high-energy phosphate donor
质量水平
方案
≥85% (elemental analysis)
表单
powder
mp
>300 °C (lit.)
溶解性
water: 25 mg/mL, clear, colorless
储存温度
−20°C
SMILES字符串
[Li+].[K+].CC(=O)OP([O-])([O-])=O
InChI
1S/C2H5O5P.K.Li/c1-2(3)7-8(4,5)6;;/h1H3,(H2,4,5,6);;/q;2*+1/p-2
InChI key
RLQMPLKXFIXRCV-UHFFFAOYSA-L
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应用
乙酰磷酸锂钾：
- 已在X射线晶体学中用作 CpxR（转录因子）底物，以浸泡CpxR受体结构域(CpxRRD)晶体实现磷酸化
- 可用作筛选研究中的磷酸盐(P)供体，结合多种酶从种羟基化合物中筛选各类天然和合成有机磷酸盐供体，改进磷酸单酯的酶促合成
- 已用于构建用于体外翻译反应的预混物A
生化/生理作用
乙酰磷酸钾锂通常用作乙酰磷酸的来源。因此，在磷酸酶催化的转磷酸化反应中，乙酰磷酸盐可作为无机低聚磷酸盐的适宜替代品，减少无机单磷酸盐副产物形成。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Repr. 2 - Skin Corr. 1B
储存分类代码
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Soma Mukhopadhyay et al.
Bioorganic chemistry, 36(2), 65-69 (2008-02-26)
Acetate kinase, a member of the ASKHA (Acetate and Sugar Kinases, Hsp70, Actin) phosphotransferase superfamily is a central enzyme in prokaryotic carbon and energy metabolism. Recently extensive structural and biochemical studies of acetate kinase and related carboxylate kinases have been
Ariel E Mechaly et al.
Structure (London, England : 1993), 25(6), 939-944 (2017-05-30)
Bacterial two-component systems consist of a sensor histidine kinase (HK) and a response regulator (RR). HKs are homodimers that catalyze the autophosphorylation of a histidine residue and the subsequent phosphoryl transfer to its RR partner, triggering an adaptive response. How
Gábor Tasnádi et al.
Advanced synthesis & catalysis, 360(12), 2394-2401 (2018-10-20)
Undesired product hydrolysis along with large amounts of waste in form of inorganic monophosphate by-product are the main obstacles associated with the use of pyrophosphate in the phosphatase-catalyzed synthesis of phosphate monoesters on large scale. In order to overcome both
Birgit Dreier et al.
Methods in molecular biology (Clifton, N.J.), 687, 283-306 (2010-10-23)
The selection and concomitant affinity maturation of proteins to bind to user-defined target molecules have become a key technology in biochemical research, diagnostics, and therapy. One of the most potent selection technologies for such applications is ribosome display. It works
Juan Andres Imelio et al.
Bio-protocol, 7(16), e2510-e2510 (2017-08-20)
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli, ensuring an excess of the regulator
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