C2014
胶原酶 来源于溶组织梭菌
0.2 μm filtered, Type V-S, ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid
别名:
梭菌肽酶 A
产品名称
胶原酶 来源于溶组织梭菌, 0.2 μm filtered, Type V-S, ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid
sterility
0.2 μm filtered
form
lyophilized powder
specific activity
≥1 FALGPA units/mg solid
≥125 CDU/mg solid
mol wt
68-125 kDa
storage temp.
−20°C
Quality Level
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Analysis Note
也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
Application
胶原酶已被用于制备动脉组织，用于研究晚期糖基化终产物（AGE）。该酶还与其他蛋白酶一起用于人肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。它在肝脏和肾脏灌注研究，胰腺消化和非实质肝细胞的分离中也是有效的。
Biochem/physiol Actions
有效地从组织中释放细胞需要胶原酶和中性蛋白酶的共同作用。每摩尔酶用 4 克原子钙 (Ca2+) 激活胶原酶。一般认为培养滤液至少含有 7 种不同的蛋白酶，分子量范围为 68-130 kDa（最适用 pH：6.3-8.8）。该酶识别序列-R-Pro-8-X-Gly-Pro-R-，其中 X 是最常见的中性氨基酸。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
Other Notes
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
Preparation Note
V 型制备 (C9263)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
Beware of the small finger and/or the proximal interphalangeal joint? Skin lacerations following collagenase injection in dupuytren contracture.
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Ask the doctor. I have Dupuytren's contracture in both hands. I've had "needle" surgery in one hand, and the fingers straightened. But they seem to want to contract again. What can I do?
Celeste Robb-Nicholson
Harvard women's health watch, 19(5), 7-7 (2012-03-09)
Mako Kato et al.
International journal of pharmaceutics, 423(2), 428-434 (2011-12-27)
Elevated interstitial fluid pressure (IFP) in a tumor is a barrier to tumor accumulation of systemic delivery of nanocarriers. In this study, we investigated whether intravenous injection of type I collagenase (collagenase-1) reduced IFP in tumors and increased the accumulation
Ernest Azzopardi et al.
British journal of hospital medicine (London, England : 2005), 73(8), 432-436 (2012-08-10)
Dupuytren's disease is a common, costly and recurrent health issue. This review compares Clostridium histolyticum collagenase with current operative treatments. Collagenase management is an effective non-surgical alternative associated with lower risks of serious adverse events, but higher incidence of non-serious
Thomas J Edkins et al.
Journal of pharmaceutical and biomedical analysis, 70, 408-414 (2012-08-03)
This paper summarizes the development and validation of five enzyme activity methods to assess the specific inhibition of human endogenous matrix metalloproteinases MMP-1 (interstitial collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin 1), MMP-8 (collagenase 2) and MMP-13 (collagenase 3) by anti-Collagenase
实验方案
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
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