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安全信息

D9905

Sigma-Aldrich

Denhardt′s溶液

for Northern and Southern blotting, lyophilized powder

别名:

用于印迹技术的Denhardt′s 溶液

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About This Item

UNSPSC代码:
12161700
NACRES:
NA.25

等级

for molecular biology

质量水平

100

表单

lyophilized powder

技术

Southern blotting: suitable

异质活性

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

储存温度

2-8°C

相关类别

一般描述

Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。

应用

Denhardt溶液已被用于：
  • 作为杂交缓冲液的组分，用于脑切片的杂交
  • 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
  • 用于杂交混合物的制备

适合用于Southern和Northern印迹膜的封闭缓冲液。

生化/生理作用

Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。

组分

每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。

制备说明

制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

含少量动物源组分生物产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCH1455
SLCB0735
SLBW1763
SLBR5407V
SLBR7277V

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Andreas Toegl et al.
Journal of biomolecular techniques : JBT, 14(3), 197-204 (2003-09-19)
Protein and DNA microarrays have become a standard tool in proteomics/genomics research. In order to guarantee fast and reproducible hybridization results, the diffusion limit must be overcome. Surface acoustic wave (SAW) micro-agitation chips efficiently agitate the smallest sample volumes (down
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
Nancy Stralis-Pavese et al.
Nature protocols, 6(5), 609-624 (2011-04-30)
Microbial diagnostic microarrays (MDMs) are highly parallel hybridization platforms containing multiple sets of immobilized oligonucleotide probes used for parallel detection and identification of many different microorganisms in environmental and clinical samples. Each probe is approximately specific to a given group
M A Hull et al.
Gut, 43(4), 525-536 (1998-11-21)
Basic fibroblast growth factor (bFGF) promotes angiogenesis and healing of gastric ulcers in rats, and bFGF expression is up regulated in such ulcers. However, little is known about expression of bFGF in human gastric mucosa. To investigate bFGF expression in
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