MDQ1
Imprint® 甲基化 DNA 定量试剂盒
To measure global DNA methylation shifts from as low as 10 ng DNA
别名:
DNA Methylation Quantification, DNA methylation shift
General description
Imprint™ 甲基化 DNA 定量技术提供了一种快速可靠的方法来测量全局 DNA 甲基化移位。印记甲基化 DNA 定量试剂盒包含检测相对水平的甲基化 DNA 所需的所有试剂。最多 200 ng 纯化 DNA 与检测试剂条的微孔结合。使用捕获和检测抗体检测甲基化 DNA，然后用比色法定量。样本中存在的甲基化 DNA 的量与测量的吸光度成正比。
Application
Imprint® 甲基化 DNA 定量试剂盒已用于全局 DNA 甲基化分析。
Features and Benefits
- 基于 ELISA 的格式
- 在 4 个易于遵循的步骤中完成程序
- 提供的所有试剂，包括对照甲基化 DNA
- 检测限为 5 ng 全甲基化 DNA
- 输入 DNA 可能低至 10-200 ng
- 程序在 3.5 小时内完成
- 96 孔格式允许研究单样本或高通量研究
- 对 5-mC 具有高特异性的优化抗体和试剂；对非甲基化胞嘧啶无交叉反应
- 可与贴壁和悬浮细胞、组织、血浆及其他体液如唾液、尿液和血清一起使用
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
wgk
WGK 3
存储类别
10 - Combustible liquids
法规信息
监管及禁止进口产品
此项目有
Roman Thaler et al.
The British journal of nutrition, 101(5), 743-749 (2008-08-08)
The impact of nutrition on the epigenetic machinery has increasingly attracted interest. The aim of the present study was to demonstrate the effects of various diets on methylation and gene expression. The antioxidative enzyme mitochondrial superoxide dismutase (MnSOD) was chosen
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Maiko Suzuki et al.
Molecular medicine reports, 1(4), 465-471 (2008-07-01)
Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis in oral squamous cell carcinoma (OSCC). In this study, we used the novel DNA methyltransferase inhibitor zebularine (Zeb) to investigate epigenetic influences on the secretion of VEGF-A in the
Alok Mishra et al.
Methods in molecular biology (Clifton, N.J.), 863, 15-31 (2012-02-24)
Epigenetics is an emerging science that can help to explain carcinogenesis. The possibility that carcinogenesis may originate in a stem cell process was proposed recently. Stem cells are generated and contribute to tumor formation during the process of tumor development.
Giulia F Del Gobbo et al.
Clinical epigenetics, 10, 34-34 (2018-03-23)
5,10-Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in one-carbon metabolism that ensures the availability of methyl groups for methylation reactions. Two single-nucleotide polymorphisms (SNPs) in the In 303 placentas screened for this study, we observed no significant association between the
商品
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
实验方案
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持