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用途
sufficient for 70 purifications
技术
DNA purification: suitable
储存温度
15-25°C
相关类别
一般描述
应用
特点和优势
- 可在8分钟内纯化出多达100 μl或10 μg 的PCR扩增产物DNA。
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可去除99%以上的引物和其他成分
- 不需要去除有机萃取引入的矿物油
- 相对于其他市场领先竞争对手，可多获得40%的纯化制备物
其他说明
原理
法律信息
警示用语：
Warning
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
Can Product No. NA1020, GenElute™ PCR Clean-Up Kit, be used to clean up PCR products larger than 10 kb?
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.
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