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Merck
CN

P8170

Sigma-Aldrich

Protein G−Peroxidase from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

别名:

Protein G−HRP from Streptococcus sp.

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关于此项目

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46
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重组

expressed in unspecified host

质量水平

偶联物

peroxidase conjugate

表单

lyophilized powder

技术

direct ELISA: 1:100,000 using human IgG

储存温度

−20°C

一般描述

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.

应用

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

生化/生理作用

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

制备说明

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Santos B S A S et al.
RSC advances, 12(49), 31711-31728 (2022-11-17)
The current COVID-19 pandemic presents one of the greatest challenges in human history. There is a consensus that the rapid and accurate diagnosis of COVID-19 directly affects procedures to avoid dissemination, promote treatments, and favor the prognosis of infected patients.
Dimitra Zarafeta et al.
Frontiers in microbiology, 7, 1779-1779 (2016-12-03)
Lipolytic enzymes that retain high levels of catalytic activity when exposed to a variety of denaturing conditions are of high importance for a number of biotechnological applications. In this study, we aimed to identify new lipolytic enzymes, which are highly
Evaluation of a modified Rose Bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep
Ferreira AC, et al.
Veterninary Research, 34(3), 297-305 (2003)
Thiol peroxidase is an important component of Streptococcus pneumoniae in oxygenated environments
Hajaj B, et al.
Infection and Immunity, IAI-00126 (2012)
Iraia Ladero-Auñon et al.
NPJ vaccines, 6(1), 102-102 (2021-08-14)
Mycobacterium avium subsp. paratuberculosis (Map) causes paratuberculosis (PTB), a granulomatous enteritis in ruminants that exerts high economic impact on the dairy industry worldwide. Current vaccines have shown to be cost-effective against Map and in some cases confer beneficial non-specific effects

实验方案

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

建立以 2,2'-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)为基质的过氧化物酶测定方法。

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