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Merck
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主要文件

安全信息

SAB1306078

Millipore

抗 FLAG标签 (DYKDDDDK) 兔抗

IgG fraction of antiserum, buffered aqueous solution

别名:

抗 ddddk, 抗 dykddddk

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.43
克隆:
polyclonal
application:
WB
种属反应性:
all
技术:
western blot: 1:1000
citations:
10

生物来源

rabbit

偶联物

unconjugated

抗体形式

IgG fraction of antiserum

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

种属反应性

all

浓度

(see Certificate of Analysis)

技术

western blot: 1:1000

同位素/亚型

IgG

UniProt登记号

运输

wet ice

储存温度

−20°C

一般描述

FLAG是由Asp-Try-Lys-Asp-Asp-Asp-Asp-Lys氨基酸序列组成的八肽。

应用

兔抗-FLAG标签(DYKDDDDK)抗体已用于免疫印迹分析, (1:5000), (1:10000)。

生化/生理作用

FLAG广泛用作融合蛋白免疫亲和纯化的表位标签。

外形

在含有 0.09% (W/V) 叠氮化钠的 PBS 中提供

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储存分类代码

12 - Non Combustible Liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Sunwang Xu et al.
Nature communications, 10(1), 5492-5492 (2019-12-04)
Gemcitabine is the first-line treatment for locally advanced and metastatic gallbladder cancer (GBC), but poor gemcitabine response is universal. Here, we utilize a genome-wide CRISPR screen to identify that loss of ELP5 reduces the gemcitabine-induced apoptosis in GBC cells in
Zhe Wang et al.
JCI insight, 4(1) (2019-01-11)
Deposition of amyloid-β protein (Aβ) to form neuritic plaques is the characteristic neuropathology of Alzheimer's disease (AD). Aβ is generated from amyloid precursor protein (APP) by β- and γ-secretase cleavages. BACE1 is the β-secretase and its inhibition induces severe side
Feiran Zhang et al.
Human molecular genetics, 27(22), 3936-3950 (2018-08-15)
N6-methyladenosine (m6A) is the most prevalent internal modification of mammalian messenger RNAs (mRNAs) and long non-coding RNAs. The biological functions of this reversible RNA modification can be interpreted by cytoplasmic and nuclear 'm6A reader' proteins to fine-tune gene expression, such
Renxu Chang et al.
Nature communications, 9(1), 3486-3486 (2018-08-30)
Loss of WW domain-containing oxidoreductase (Wwox) expression has been observed in breast cancer (BC). However, its regulatory effects are largely unknown, especially in triple-negative breast cancer (TNBC). Herein, gene expression profiling revealed that JAK/STAT3 pathway was one of the most
Yajie Gao et al.
STAR protocols, 3(3), 101596-101596 (2022-08-06)
Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation of RNA probes, the target

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