06-926
Anti-p70 S6 Kinase (S6K) Antibody
Upstate®, from rabbit
别名:
RPS6KB1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha, ribosomal protein S6 kinase, 70kDa
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Species reactivity:
rat, human, mouse
Application:
WB
Citations:
8
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
Gene Information
human ... POTEM(641455)
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
64kDa
Immunogen
peptide (C-FPMISKRPEHLRMNL) corresponding to residues 511-525 of rat S6 kinase
Application
Anti-p70 S6 Kinase (S6K) Antibody detects level of p70 S6 Kinase (S6K) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Biochem/physiol Actions
p70 S6 kinase
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the additional of glycerol to 30%
Format: Purified
Protein A chromatography
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from mouse 3T3 cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 1
Activation of an S6 kinase from human placenta by autophosphorylation.
Dennis, P B and Masaracchia, R A
The Journal of Biological Chemistry, 268, 19833-19841 (1993)
SWI/SNF chromatin remodeling enzyme ATPases promote cell proliferation in normal mammary epithelial cells.
Cohet, N; Stewart, KM; Mudhasani, R; Asirvatham, AJ; Mallappa, C; Imbalzano, KM; Weaver et al.
Journal of Cellular Physiology null
Quan-Yu Zhang et al.
Hepatology (Baltimore, Md.), 66(3), 834-854 (2017-05-17)
Cellular repressor of E1A-stimulated genes (CREG), a novel cellular glycoprotein, has been identified as a suppressor of various cardiovascular diseases because of its capacity to reduce hyperplasia, maintain vascular homeostasis, and promote endothelial restoration. However, the effects and mechanism of
Tingting Sun et al.
Aging, 12(23), 24208-24218 (2020-11-24)
The 5-year survival rate of ovarian cancer patients is only 47%, and developing novel drugs for ovarian cancer is needed. Herein, we evaluated if and how SRT2183, a sirtuin-1 activator, impairs the ovarian cancer cells. OVCAR-3 and A2780 cells were
D Chen et al.
Molecular and cellular biology, 19(7), 4684-4694 (1999-06-22)
We have previously reported that insulin and osmotic shock stimulate an increase in glucose transport activity and translocation of the insulin-responsive glucose transporter isoform GLUT4 to the plasma membrane through distinct pathways in 3T3L1 adipocytes (D. Chen, J. S. Elmendorf
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