53702
Pronase®蛋白酶，灰色链霉菌
别名:
Pronase® powder
description
RTECS - UK9595000
form
lyophilized
specific activity
≥45,000 proteolytic units/g dry wt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
solubility
water: 10 mg/mL
shipped in
ambient
storage temp.
2-8°C
Quality Level
General description
一种非特异性蛋白酶将粘蛋白液化并消化蛋白质以释放氨基酸。可用于分离活的软骨细胞。在中性pH下最具活性，但可在较宽的pH和温度范围内稳定。最适pH为7.5。注意：1 KU = 1000单位。
注意：1 KU = 1000单位。
Physical form
从含有乙酸钙作为稳定剂的溶液进行冻干。
Preparation Note
在重悬后存放于冰箱（4°C）。储备液可在4°C稳定24小时。
Other Notes
Narahashi, Y., et al. 1968.J. Biochem.64, 427.
一个单位的定义为在40°C、pH 7.5下每分钟释放25 µg酪氨酸当量消化产物所需的酶量。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：有害（C）
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
The preparation and characterization of cell walls and the preparation of flagella of Vibrio fetus.
R F Keeler et al.
Journal of general microbiology, 43(3), 439-454 (1966-06-01)
T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
Nicole M Templeman et al.
Developmental cell, 54(1), 92-105 (2020-06-17)
Evolutionarily conserved signaling pathways are crucial for adjusting growth, reproduction, and cell maintenance in response to altered environmental conditions or energy balance. However, we have an incomplete understanding of the signaling networks and mechanistic changes that coordinate physiological changes across
Aimee E Stephenson et al.
Molecular microbiology, 43(1), 147-157 (2002-02-19)
Streptococcus parasanguis is a primary colonizer of the tooth surface and plays a pivotal role in the formation of dental plaque. The fimbriae of S. parasanguis are important in mediating adhesion to saliva-coated hydroxylapatite (SHA), an in vitro tooth adhesion
Kenya Terabe et al.
The Journal of biological chemistry, 294(47), 17799-17817 (2019-10-18)
Hyaluronan is a critical component of articular cartilage and partially helps retain aggrecan within the extracellular matrix of this tissue. During osteoarthritis, hyaluronan and aggrecan loss are an early sign of tissue damage. However, our recent attempts to mimic hyaluronan
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