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Merck
CN

53702

Pronase®蛋白酶,灰色链霉菌

别名:

Pronase® powder

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化学文摘社编号:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
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description

RTECS - UK9595000

form

lyophilized

specific activity

≥45,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

solubility

water: 10 mg/mL

shipped in

ambient

storage temp.

2-8°C

Quality Level

Disclaimer

毒性:有害(C)

General description

一种非特异性蛋白酶将粘蛋白液化并消化蛋白质以释放氨基酸。可用于分离活的软骨细胞。在中性pH下最具活性,但可在较宽的pH和温度范围内稳定。最适pH为7.5。注意:1 KU = 1000单位。
注意:1 KU = 1000单位。

Other Notes

Narahashi, Y., et al. 1968.J. Biochem.64, 427.
一个单位的定义为在40°C、pH 7.5下每分钟释放25 µg酪氨酸当量消化产物所需的酶量。

Physical form

从含有乙酸钙作为稳定剂的溶液进行冻干。

Preparation Note

在重悬后存放于冰箱(4°C)。储备液可在4°C稳定24小时。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 2


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T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
The preparation and characterization of cell walls and the preparation of flagella of Vibrio fetus.
R F Keeler et al.
Journal of general microbiology, 43(3), 439-454 (1966-06-01)
Nicole M Templeman et al.
Developmental cell, 54(1), 92-105 (2020-06-17)
Evolutionarily conserved signaling pathways are crucial for adjusting growth, reproduction, and cell maintenance in response to altered environmental conditions or energy balance. However, we have an incomplete understanding of the signaling networks and mechanistic changes that coordinate physiological changes across
Jun Sunayama et al.
Stem cells (Dayton, Ohio), 28(11), 1930-1939 (2010-09-22)
The molecular signaling pathways orchestrating the biology of cancer stem-like cells (CSLCs), including glioblastoma, remain to be elucidated. We investigated in this study the role of the MEK/extracellular signal-regulated kinase (ERK) pathway in the control of self-renewal and tumorigenicity of
Yifei Jiang et al.
Anesthesiology, 125(6), 1159-1170 (2016-09-23)
Exposure to isoflurane increases apoptosis among postnatally generated hippocampal dentate granule cells. These neurons play important roles in cognition and behavior, so their permanent loss could explain deficits after surgical procedures. To determine whether developmental anesthesia exposure leads to persistent

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