产品名称
Human TNF-α Conferma® ELISA Kit,
Quality Segment
description
kit sufficient for 96 wells
species reactivity
human
packaging
kit of 1 × 96 wells
parameter
50 μL sample volume (4hr assay)
technique(s)
ELISA: suitable
input
sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)
application(s)
research use
assay range
linearity: 89-113%
(Serum, 101%), linearity: 98-110%
(Plasma, 103%), recovery range: 81-111%
(Plasma, 97%), recovery range: 85-105%
(Serum,95%), inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
General description
TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.
Research Category: Inflammation
Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease
Research Category: Inflammation
Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease
Application
For the detection of TNF-α by ELISA in Human Serum & Plasma.
Biochem/physiol Actions
This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.
Features and Benefits
Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.
Legal Information
CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
存储类别
8A - Combustible, corrosive hazardous materials
wgk
WGK 3


