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P1379

Sigma-Aldrich

TWEEN ® 20

viscous liquid

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别名:
聚氧乙烯山梨糖醇酐单月桂酸酯, 聚氧乙烯脱水山梨醇单月桂酸酯
CAS号:
9005-64-5
MDL编号:
MFCD00165986
NACRES:
NA.21

描述

non-ionic

质量水平

300

检测方案

>40% (GC)

形式

viscous liquid

分子量

~1228

组成

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

浓度

≥40.0% (GC)

技术

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

杂质

≤3.0% water

折射率

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

酸值

≤2.2 mg/g

羟基值

96‑108 mg/g

转变温度

cloud point 76 °C

密度

1.095 g/mL at 25 °C (lit.)

HLB

16.7

适用性

complies for IR spectroscopy

SMILES字符串

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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相关类别

一般描述

Tween®20是属于聚山梨醇酯家族的一种聚氧乙烯山梨糖醇酯。它是一种分子量为1,225道尔顿的非离子型去污剂，假设有20个环氧乙烷单位、1个山梨糖醇和1个月桂酸作为主要脂肪酸。环氧乙烷亚基负责表面活性剂的亲水性，而烃链则提供了疏水环境。山梨糖醇可形成连接了环氧乙烷聚合物的骨架环。
吐温® 20 是一种非离子表面活性剂和去污剂，广泛应用于生物化学和生命科学研究。其主要用途涉及膜-蛋白复合物分离过程中膜蛋白的增溶。 此外，它可作为各种清洗、封闭、稀释和稳定缓冲液的成分。另外，它可用作免疫组织化学（包括蛋白质印迹和ELISA等技术）的试剂。在这些应用中，吐温® 20起到关键作用，例如防止非特异性抗体结合，最大程度减少背景染色，确保试剂的均匀分布。

表面活性剂同时具有亲水和疏水成分，可以分为两大类：在水中电离的离子型（阴离子、阳离子或两性）和不电离的非离子型 。所述产品为非离子表面活性剂，可用于各种应用，如乳化、分散、增溶和去污剂。此外，它可用作膜蛋白的增溶剂。

应用

TWEEN 20作为组分已用于：
  • Western blotting中的洗涤和封闭缓冲液
  • 免疫组化中的封闭缓冲液
  • ELISA（酶联免疫吸附测定）中的洗涤缓冲液
  • PCR（聚合酶链反应）中的反应混合液
TWEEN 20是一种广泛用于生化应用的非离子型去污剂。它已被作为乳化剂用于稳定的水包油乳液的制备。TWEEN 20已用于膜的预提取以去除外周蛋白（以2％用于膜结合蛋白的提取）。
通常0.05%浓度的TWEEN 20在基于膜的免疫检测中被用作封闭剂。0.005至0.5%的TWEEN 20可用于裂解哺乳动物细胞。
非离子洗涤剂

特点和优势

  • 用于生命科学和生物化学研究的多功能表面活性剂
  • 良好的增溶、分散和乳化剂
  • 适用于DNA & RNA 提取、哺乳动物细胞培养和蛋白质纯化

包装

P1379-1KT：
每个试剂盒包含3 x 100ML样品，每份样品都有唯一的制造商批号。

制备说明

TWEEN 20可与水混溶（100mg/mL），产生透明的黄色溶液。它还可与酒精、二恶烷和乙酸乙酯进行混溶。TWEEN 20不溶于液体石蜡和固定油。

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

TWEEN is a registered trademark of Croda International PLC

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

527.0 °F - Pensky-Martens closed cup

闪点(°C)

275 °C - Pensky-Martens closed cup

个人防护装备

Eyeshields, Gloves

分析证书(COA)

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  1. What is the percentage of Tween 20 in Product P1379?

    P1379 is not diluted in any solvent. Thus it can be considered "100%" or the "neat" material.

  2. Can solutions of Product P1379, Tween® 20, be autoclaved?

    Increased temperature can lead to autoxidation of TWEEN 20 in solution. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. TWEEN 20 is heat sensitive; it will darken when exposed to elevated temperatures. Autoclaving is done at the customer's risk.

  3. What is the source of Product P1379, Tween® 20? Is it from plant or animal source, or synthetic?

    Origin information on any lot of Tween 20 is specific to that lot, and can be found on its Certificate of Origin (CofO), which may be accessed on the Sigma-Aldrich website under its specific product and lot number.

  4. The appearance of Product P1379, Tween® 20, is clear dark yellow. Is this OK?

    Yes. The specifications for P1379 are "clear yellow to yellow green viscous liquid".

  5. Is Product P1379, Tween® 20, protease-free?

    We have not tested P1379 in quality control to be protease-free. There is a Molecular Biology grade of Tween 20, P9416, but this is not tested specifically for proteases either. P9416 is tested to be endonuclease-endonuclease free and to be ribonuclease-free.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.
Kerwin BA
J. Pharm. Pharm. Sci., 97(8), 2924-2935 (2008)
Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification.
Seal SE et al
Applied and Environmental Microbiology, 58(11), 3759-3761 (1992)
Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle.
Gr?nevik E et al
The journal of gene medicine, 7(2), 218-227 (2005)
Ethylene and carbon dioxide production by developing strawberries show a correlative pattern that is indicative of ripening climacteric fruit.
Lannetta PM et al
Physiologia Plantarum, 127.2, 247-259 (2006)
Jennifer Somerfield et al.
Journal of immunology (Baltimore, Md. : 1950), 185(1), 763-768 (2010-06-04)
Biological therapies, even humanized mAbs, may induce antiglobulin responses that impair efficacy. We tested a novel strategy to induce tolerance to a therapeutic mAb. Twenty patients with relapsing-remitting multiple sclerosis received an initial cycle of alemtuzumab (Campath-1H), up to 120
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