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B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

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别名:
Sodium Borate Electrophoresis Buffer
NACRES:
NA.25

等级

for molecular biology

质量水平

200

形式

solution

浓度

10 ×

杂质

DNase, RNase and nickase, none detected

pH值（酸碱度）

8.0-8.5 (20-25 °C)

储存温度

room temp

相关类别

一般描述

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

应用

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

特点和优势

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

包装

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

注意

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

法律信息

Bionic is a trademark of Sigma-Aldrich Co. LLC

象形图

Exclamation markHealth hazard
GHS07,GHS08

警示用语：

Danger

危险声明

H319 - H360FD

危险分类

Eye Irrit. 2 - Repr. 1B

储存分类代码

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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Maria Desemparats Saenz-de-Juano et al.
Biology of reproduction, 87(4), 91-91 (2012-08-03)
Studies of embryo cryopreservation efficiency have focused mainly on technical and embryo factors. To determine how a slow freezing process affects embryo and fetal development, we studied in vivo development ability after the freezing procedure by assessing blastocyst development at
Fast short-fragment PCR for rapid and sensitive detection of shrimp viruses.
Mrotzek G, et al.
Journal of Virological Methods, 168, 262-266 (2010)
Carmen Naturil-Alfonso et al.
PloS one, 7(12), e51271-e51271 (2012-12-20)
Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate

实验方案

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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