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安全信息

B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

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别名:
Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside
经验公式（希尔记法）:
C14H15BrClNO6
CAS号:
93863-88-8
分子量:
408.63
MDL编号:
MFCD02683897
PubChem化学物质编号:
329773145
NACRES:
NA.85

等级

for molecular biology

质量水平

200

无菌性

non-sterile

产品线

BioReagent

检测方案

≥98.0% (HPLC)

形式

powder

溶解性

methanol: soluble

适用性

suitable for substrate for β-galactosidase

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1

InChI key

CHRVKCMQIZYLNM-HTOAHKCRSA-N

一般描述

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

应用

Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.
Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.

原理

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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