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安全信息

C4482

Sigma-Aldrich

CelLytic ™ Y 细胞裂解试剂

For yeast cells

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别名:
Yeast cell lysis reagent
NACRES:
NA.56

质量水平

200

形式

solution

相关类别

一般描述

CelLytic Y 细胞裂解试剂具有通用性、无磷酸盐和非变性。为细胞裂解和蛋白溶解提供了有效的方法。维持免疫反应性或生物学功能的靶蛋白。方案简短，在室温下执行。无需极端条件或玻璃珠。仅使用 2.5-5 mL/g 酵母细胞。

应用

CelLytic ™ Y 细胞裂解试剂已用于裂解表达 DNA 修复、Rad 蛋白、 热休克蛋白 (ssa1) 和人白细胞介素 4 (hIL4) 的酵母细胞。

特点和优势

  • 快速：在 20 min 内实现细胞裂解
  • 效率：比玻璃珠高三倍
  • 柔性：加入还原剂 (DTT) 后裂解增强
  • 温和：非变性-保留生物活性

法律信息

CelLytic is a trademark of Sigma-Aldrich Co. LLC

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H319 - H412

危险分类

Aquatic Chronic 3 - Eye Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

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  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

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部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    If cell growth conditions are not optimal, then glass beads can be used to increase yields.

  4. Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?

    For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent.  Addition of DTT significantly improves total protein yield.

  5. Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?

    No.  For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.

  6. What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?

    The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.

  7. What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)

商品

Yeast Cell Lysis Reagents

Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris and Schizosaccharomyces pombe yeast strains.

酵母细胞裂解试剂

我们的酵母细胞裂解试剂具有通用性、无磷酸盐、非变性等特点。CelLytic Y细胞裂解试剂可作为酵母细胞裂解和蛋白溶解的有效方法。CelLytic Y酵母酶解试剂盒为从酿酒酵母、巴斯德毕赤酵母和粟酒裂殖酵母酵母菌株中高效形成原生质球和提取蛋白提供了一种便捷的方法。

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

相关内容

蛋白提取_细胞裂解_蛋白质生物学-默克生命科学

细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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