产品名称
BL21(DE3)电感受态细胞, for protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
BL21（DE3）电转感受态细胞是第一个在相同细胞中实现高效克隆和高水平蛋白质表达的细胞。
克隆效率比其它 BL21 细胞制剂提高了 25-1,000 倍，这对于构建复杂的表达文库至关重要。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
克隆效率比其它 BL21 细胞制剂提高了 25-1,000 倍，这对于构建复杂的表达文库至关重要。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
BL21（DE3）电转感受态细胞（>5 × 109 cfu/μg）前所未有的转化效率消除了将质粒从克隆菌株转移到表达菌株的需要，从而为典型的克隆和表达实验节省了数天的工作时间。
Other Notes
- BL21（DE3）电转感受态细胞
- pUC19转化对照DNA
- 表达恢复培养基
存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
新产品
此项目有
实验方案
Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.
Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Zev A Ripstein et al.
eLife, 9 (2020-01-10)
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CMC0016-4X25UL | 04061838042460 |
| CMC0016-20X25UL | 04061838042439 |
| CMC0016-20X100UL | 04061838042422 |
| CMC0016-20X50UL | 04061838042446 |
| CMC0016-40X25UL | 04061838042453 |