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D0426

DAB with Metal Enhancer

Name: DAB with Metal Enhancer
Brand: SIGMA

SIGMAFAST^™, peroxidase substrate, tablet

别名:

3,3′-二氨基联苯胺过氧化物酶底物, DAB过氧化物酶底物

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关于此项目

UNSPSC Code:

12352204

NACRES:

NA.83

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产品名称

SIGMAFAST^™ DAB，含金属增强剂, tablet

form

tablet

Quality Level

200

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

General description

SIGMA FAST^™ DAB片剂（含金属增强剂）可直接制备用于过氧化物酶活性检测的活性底物溶液，无需额外的缓冲液或步骤。常用作免疫组织学的沉淀底物。

Application

含金属强化剂的SIGMAFAST^™ DAB 已被用于通过免疫组织化学观察肺组织切片、背根神经节（DRG）神经元和前十字韧带（ACL）细胞中胶原蛋白的免疫组织化学染色。

通过添加CoCl₂可以增强DAB反应，并产生更强的耐酒精色斑。

Preparation Note

将每个片剂组合溶于5 mL去离子水中，得到含有DAB和金属强化剂的即用型缓冲溶液。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS08,GHS05,GHS09

signalword

Danger

hcodes

H317,H318,H334,H341,H350,H360F,H411

pcodes

P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Apoptotic mechanism behind the testicular atrophy in photorefractory and scotosensitive quail: Involvement of GnIH induced p-53 dependent Bax-Caspase-3 mediated pathway.

Somanshu Banerjee et al.

Journal of photochemistry and photobiology. B, Biology, 176, 124-135 (2017-10-11)

In most of the avian species, daylength or photoperiod is the main environmental factor regulating reproduction. During their annual gonadal cycle, birds once sensitive to short or long day effect develop refractoriness to the same daylength and gonad develop or

Developing rat lung has a sided pacemaker region for morphogenesis-related airway peristalsis

Jesudason EC, et al.

American Journal of Respiratory Cell and Molecular Biology, 32(2), 118-127 (2005)

Cdk5 inhibitor roscovitine alleviates neuropathic pain in the dorsal root ganglia by downregulating N-methyl-D-aspartate receptor subunit 2A

Yang L, et al.

Neurological Sciences, 35(9), 1365-1371 (2014)

Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture

Cheng M, et al.

Tissue Engineering: Part A, 16(5), 1479-1489 (2010)

Evaluation of Hematobin as a Vaccine Candidate to Control Haematobia irritans (Diptera: Muscidae) Loads in Cattle.

M Breijo et al.

Journal of economic entomology, 110(3), 1390-1393 (2017-04-08)

The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated.

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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

实验方案

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

全球贸易项目编号

货号	GTIN
D0426-5SET	04061835556427
D0426-50SET	04061835506187

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主要文件

DAB with Metal Enhancer

SIGMAFAST^™, peroxidase substrate, tablet