产品名称
杜氏磷酸盐缓冲盐水, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
2-8°C
General description
杜氏磷酸盐缓冲盐水（D-PBS）是一种用于组织培养的平衡盐溶液。
Application
D-PBS是通用的等渗盐水溶液，用于在制备和连续转移过程中洗涤细胞。
杜氏磷酸盐缓冲盐水（D-PBS）已用于：
- 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
- 在Caco-2BBe细胞经上皮电阻（TEER）测量后清洗和平衡细胞
- 清洗细胞以进行蛋白质分离
杜氏磷酸盐缓冲盐水（D-PBS）已用于：
- 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
- 在Caco-2BBe细胞经上皮电阻（TEER）测量后清洗和平衡细胞
- 清洗细胞以进行蛋白质分离
Biochem/physiol Actions
杜氏磷酸盐缓冲盐水（D-PBS）有四种主要用途：
- 为细胞提供正常细胞代谢所必需的水分和一定体积的无机离子
- 充当灌溉、运输和稀释液体，同时维持细胞内和细胞外渗透平衡
- 与碳水化合物（如葡萄糖）结合时，为细胞代谢提供主要的能量来源
- 提供缓冲系统，以维持培养基的生理pH值范围
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
实体瘤以三维(3D)空间构象生长，导致氧和营养物质以及其他物理和化学应力的异质性暴露。为了模拟三维空间构象，在肿瘤研究中普遍使用三 维体外培养模型。
E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
L Parmegiani et al.
Journal of assisted reproduction and genetics, 35(10), 1887-1895 (2018-08-04)
To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D8662-100ML | 04061838259417 |
| D8662-24X500ML | 04061833589786 |
| D8662-1L | 04061833589779 |
| D8662-6X1L | 04061833589809 |
| D8662-6X500ML | 04061833589816 |
| D8662-500ML | 04061833589793 |