G1N350

GenElute^™ 哺乳动物基因组DNA小量制备试剂盒

Name: GenElute<SUP>™</SUP> 哺乳动物基因组DNA小量制备试剂盒
Brand: SIGMA

sufficient for 350 purifications

别名:

哺乳动物基因组DNA小量制备, Gen Elute

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关于此项目

UNSPSC代码:

41105501

NACRES:

NA.55

主要文件

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用途

sufficient for 350 purifications

质量水平

200

技术

DNA purification: suitable

储存温度

15-25°C

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一般描述

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，当裂解物离心通过微量离心管时DNA会结合在硅胶膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A₂₆₀/A₂₈₀比率介于1.6-1.9之间，最大长度可达50 kb。

纯化所得的基因组DNA可直接用于限制性酶切、PCR、Southern印迹、测序反应等下游应用。

应用

纯化所得的基因组DNA可直接供以下下游应用使用：

限制性内切酶酶切
PCR
Southern印迹
测序反应
克隆
连接

GenElute^™ Mammalian Genomic DNA Miniprep Kits has been used:

to extract DNA from fin samples
to extract the BSTUI restriction enzyme from the DNA
to isolate DNA from the blood samples extracted from caudal fin
to extract tissue and hair follicle DNA

生化/生理作用

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A₂₆₀/A₂₈₀比率介于1.6-1.9之间，最大长度可达50 kb。

特点和优势

预期得率：25 μg / 2 x 10⁶个培养细胞；30 μg / 25 mg组织
洗脱体积：200 - 400 μl
所需时间：细胞裂解后20分钟
A₂₆₀/A₂₈₀比率：1.6 - 1.9
是否需要机械式均质化：否

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号

说明

化学品安全说明书

C2112Column Preparation Solution化学品安全说明书

P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein化学品安全说明书

R6148RNase A solution化学品安全说明书

象形图

GHS08,GHS05,GHS07,GHS09

警示用语：

Danger

危险声明

H290,H302,H315,H318,H334,H335,H410

预防措施声明

P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

低风险生物材料

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps.

Gudrun Dittrich-Schröder et al.

Molecular ecology resources, 12(1), 109-115 (2011-09-29)

DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of

Genetic shifts in the transition from wild to farmed white-tailed deer (Odocoileus virginianus) population

Hernandez-Mendoza P M, et al.

International Journal of Biodiversity Science, Ecosystem Services & Management, 10(1), 3-8 (2014)

Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico

Parra-Bracamonte G M, et al.

Electronic journal of Biotechnology, 14(6), 11-11 (2011)

Apparent lack of a domestic animal reservoir in Gambiense sleeping sickness in northwest Uganda.

Apollo Simon Peter Balyeidhusa et al.

Veterinary parasitology, 187(1-2), 157-167 (2012-01-17)

The role played by domestic animals in the transmission of gambiense Human African Trypanosomosis remains uncertain. Northwest Uganda is endemic for Trypanosoma brucei gambiense. Of the 3267 blood samples from domestic animals in four counties examined by hematocrit centrifugation technique

Human erythrocytes bind and inactivate type 5 adenovirus by presenting Coxsackie virus-adenovirus receptor and complement receptor 1.

Robert C Carlisle et al.

Blood, 113(9), 1909-1918 (2009-01-10)

Type 5 adenovirus (Ad5) is a human pathogen that has been widely developed for therapeutic uses, with only limited success to date. We report here the novel finding that human erythrocytes present Coxsackie virus-adenovirus receptor (CAR) providing an Ad5 sequestration

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实验方案

GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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GenElute^™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 350 purifications