G1N350

GenElute^™ 哺乳动物基因组DNA小量制备试剂盒

Name: GenElute<SUP>™</SUP> 哺乳动物基因组DNA小量制备试剂盒
Brand: SIGMA

sufficient for 350 purifications

别名:

哺乳动物基因组DNA小量制备, Gen Elute

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关于此项目

NACRES:

NA.55

UNSPSC Code:

41105501

主要文件

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产品名称

GenElute^™ 哺乳动物基因组DNA小量制备试剂盒, sufficient for 350 purifications

usage

sufficient for 350 purifications

technique(s)

DNA purification: suitable

storage temp.

15-25°C

Quality Level

200

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Application

GenElute^™ Mammalian Genomic DNA Miniprep Kits has been used:

to extract DNA from fin samples
to extract the BSTUI restriction enzyme from the DNA
to isolate DNA from the blood samples extracted from caudal fin
to extract tissue and hair follicle DNA

纯化所得的基因组DNA可直接供以下下游应用使用：

限制性内切酶酶切
PCR
Southern印迹
测序反应
克隆
连接

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Biochem/physiol Actions

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A₂₆₀/A₂₈₀比率介于1.6-1.9之间，最大长度可达50 kb。

Features and Benefits

预期得率：25 μg / 2 x 10⁶个培养细胞；30 μg / 25 mg组织
洗脱体积：200 - 400 μl
所需时间：细胞裂解后20分钟
A₂₆₀/A₂₈₀比率：1.6 - 1.9
是否需要机械式均质化：否

General description

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，当裂解物离心通过微量离心管时DNA会结合在硅胶膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A₂₆₀/A₂₈₀比率介于1.6-1.9之间，最大长度可达50 kb。

纯化所得的基因组DNA可直接用于限制性酶切、PCR、Southern印迹、测序反应等下游应用。

Click here to view the kit protocol

Other Notes

更多信息，请见www.sigma-aldrich.com/genomicdna。

pictograms

GHS08,GHS05,GHS07,GHS09

signalword

Danger

hcodes

H290,H302,H315,H318,H334,H335,H410

pcodes

P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000486108

0000486097

0000417770

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DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps.

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Hernandez-Mendoza P M, et al.

International Journal of Biodiversity Science, Ecosystem Services & Management, 10(1), 3-8 (2014)

Novel single nucleotide polymorphisms in candidate genes for growth in tilapia (Oreochromis niloticus)

Cuevas-Rodriguez B L, et al.

Revista Brasileira de Zootecnia, 45(6), 345-348 (2016)

Tumour necrosis factor-alpha increases extravasation of virus particles into tumour tissue by activating the Rho A/Rho kinase pathway.

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Journal of controlled release : official journal of the Controlled Release Society, 156(3), 381-389 (2011-09-03)

Tumour Necrosis Factor alpha (TNF) is a pleiotropic pro-inflammatory cytokine with known vascular permeabilising activity. It is employed during isolated limb perfusion to enhance delivery of chemotherapeutic drugs into tumour tissue. The use of conditionally-replicating lytic viruses, so called 'oncolytic

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Parra-Bracamonte G M, et al.

Electronic journal of Biotechnology, 14(6), 11-11 (2011)

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实验方案

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

查看所有结果

GenElute^™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 350 purifications