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Merck
CN

M2279

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.75

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产品名称

Eagle最低必需培养基, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

最低必需培养基（MEM）是使用最广泛的合成细胞培养基之一。它包含更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。MEM已用于在胎牛血清（FBS）、小牛或马血清环境中培养各种单层细胞和附着细胞系（如成纤维细胞）。

应用

Eagle最低必需培养基已用于培养：
  • 主动脉瓣小叶的外植体
  • 人肝癌细胞系（HepG2）细胞
  • 来源于人胎儿大脑祖细胞的星形胶质细胞（PDA）
  • 乳腺癌细胞系(MCF-7)
  • 人成胶质细胞瘤细胞系(T98G)

制备说明

补充0.292 g/L L-谷氨酰胺。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL9391
RNBL9244
RNBL7911
RNBL7297
RNBL6717

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