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Merck
CN

M2279

Eagle最低必需培养基

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75

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产品名称

Eagle最低必需培养基, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Application

Eagle最低必需培养基已用于培养：
  • 主动脉瓣小叶的外植体
  • 人肝癌细胞系（HepG2）细胞
  • 来源于人胎儿大脑祖细胞的星形胶质细胞（PDA）
  • 乳腺癌细胞系(MCF-7)
  • 人成胶质细胞瘤细胞系(T98G)

General description

最低必需培养基（MEM）是使用最广泛的合成细胞培养基之一。它包含更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。MEM已用于在胎牛血清（FBS）、小牛或马血清环境中培养各种单层细胞和附着细胞系（如成纤维细胞）。

Preparation Note

补充0.292 g/L L-谷氨酰胺。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL9391
RNBL9244
RNBL7911
RNBL7297
RNBL6717

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Winston X Yan et al.
Nature communications, 8, 15058-15058 (2017-05-13)
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS)
Kaisa-Emilia Makkonen et al.
Journal of virology, 87(20), 11148-11159 (2013-08-09)
Baculoviruses are insect-specific viruses commonly found in nature. They are not able to replicate in mammalian cells but can transduce them when equipped with an appropriate mammalian cell active expression cassette. Although the viruses have been studied in several types
Emily R Trzeciak et al.
Cells, 11(6) (2022-03-26)
The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T
Luciano A Masullo et al.
Nature communications, 9(1), 3281-3281 (2018-08-18)
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging
Anna Bodzon-Kulakowska et al.
Cytotechnology, 67(6), 1085-1091 (2014-05-08)
Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the
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